Chapter 9. Structural Genomics

  1. Dr. Albrecht Messerschmidt

Published Online: 5 JAN 2007

DOI: 10.1002/9783527610129.ch9

X-Ray Crystallography of Biomacromolecules: A Practical Guide

X-Ray Crystallography of Biomacromolecules: A Practical Guide

How to Cite

Messerschmidt, A. (2007) Structural Genomics, in X-Ray Crystallography of Biomacromolecules: A Practical Guide, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527610129.ch9

Author Information

  1. Max-Planck-Institue of Biochemistry, Department of Proteomics and Signal Transduction, Research Group: Structural Proteomics, Am Klopferspitz 18, 82152 Martinsried, Germany

Publication History

  1. Published Online: 5 JAN 2007
  2. Published Print: 15 DEC 2007

ISBN Information

Print ISBN: 9783527313969

Online ISBN: 9783527610129

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Keywords:

  • structural genomics;
  • target selection;
  • production of recombinant proteins;
  • expression systems;
  • protein purification;
  • quality control of the purified protein;
  • automation

Summary

This chapter contains sections titled:

  • Introduction

  • Target Selection

  • Production of Recombinant Proteins

    • Introduction

    • Engineering an Appropriate Expression Construct

    • Expression Systems

      • E. coli

      • Eukaryotic Expression Systems

        • Yeasts

        • Baculovirus

        • Mammalian Cells

    • Protein Purification

      • Precipitation

      • Chromatography

    • Quality Control of the Purified Protein

  • Aspects of Automation