Chapter 3. ALIS: An Affinity Selection–Mass Spectrometry System for the Discovery and Characterization of Protein–Ligand Interactions

  1. Prof. Dr. Klaus T. Wanner and
  2. Dr. Georg Höfner
  1. Allen Annis1,
  2. Cheng-Chi Chuang2 and
  3. Naim Nazef2

Published Online: 24 MAY 2007

DOI: 10.1002/9783527610907.ch3

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

How to Cite

Annis, A., Chuang, C.-C. and Nazef, N. (2007) ALIS: An Affinity Selection–Mass Spectrometry System for the Discovery and Characterization of Protein–Ligand Interactions, in Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery (eds K. T. Wanner and G. Höfner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527610907.ch3

Editor Information

  1. Ludwig-Maximilians-University Munich, Department of Pharmacy, Center of Drug Research, Butenandtstr., 81377 Munich, Germany

Author Information

  1. 1

    Schering–Plough Research Institute, 320 Bent Street, Cambridge, MA 02141, USA

  2. 2

    Schering–Plough Research Institute, 840 Memorial Drive, Cambridge, MA 02139, USA

Publication History

  1. Published Online: 24 MAY 2007
  2. Published Print: 23 FEB 2007

Book Series:

  1. Methods and Principles in Medicinal Chemistry

Book Series Editors:

  1. Prof. Dr. Raimund Mannhold4,
  2. Prof. Dr. Hugo Kubinyi5 and
  3. Prof. Dr. Gerd Folkers6

Series Editor Information

  1. 4

    Molecular Drug Research Group, Heinrich-Heine-Universität, Universitätsstrasse 1, 40225 Düsseldorf, Germany

  2. 5

    Donnersbergerstrasse 9, 67256 Weisenheim am Sand, Germany

  3. 6

    Collegium Helveticum, STW/ETH Zurich, 8092 Zurich, Switzerland

ISBN Information

Print ISBN: 9783527314560

Online ISBN: 9783527610907

SEARCH

Keywords:

  • affinity selection–mass spectrometry system for the discovery and characterization of protein–ligand interactions;
  • ALIS;
  • ALIS system design;
  • spectroscopic and biophysical methods;
  • discovery of ligands from combinatorial libraries;
  • quantitative binding affinity measurement;
  • competition-based binding site determination and affinity ranking in mixtures;
  • protein–ligand dissociation rate measurement

Summary

This chapter contains sections titled:

  • Introduction

    • State of the Art

      • Spectroscopic and Biophysical Methods

      • Mass Spectrometry-based Methods

  • ALIS: An Affinity Selection–Mass Spectrometry System based on Continuous SEC

    • ALIS System Design

  • Discovery of Ligands from Combinatorial Libraries

  • Quantitative Binding Affinity Measurement

    • Theory

    • Simulations and Experimental Results

  • Competition-based Binding Site Determination and Affinity Ranking in Mixtures

    • Binding Site Classification

    • Affinity Ranking in Compound Mixtures

  • Protein–Ligand Dissociation Rate Measurement

    • Theory

    • Simulations

    • Experimental Results

  • Conclusions

  • Future Directions