Chapter 4. Library Screening Using Ultrafiltration and Mass Spectrometry

  1. Prof. Dr. Klaus T. Wanner and
  2. Dr. Georg Höfner
  1. Timothy E. Cloutier1 and
  2. Kenneth M. Comess2

Published Online: 24 MAY 2007

DOI: 10.1002/9783527610907.ch4

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

How to Cite

Cloutier, T. E. and Comess, K. M. (2007) Library Screening Using Ultrafiltration and Mass Spectrometry, in Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery (eds K. T. Wanner and G. Höfner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527610907.ch4

Editor Information

  1. Ludwig-Maximilians-University Munich, Department of Pharmacy, Center of Drug Research, Butenandtstr., 81377 Munich, Germany

Author Information

  1. 1

    Target and Lead Discovery, Global Pharmaceutical R&D, 100 Abbott Park, Abbott Park, IL 60064, USA

  2. 2

    Abbott Labs PPD R&D, 100 Abbott Park, Abbott Park, IL 60064, USA

Publication History

  1. Published Online: 24 MAY 2007
  2. Published Print: 23 FEB 2007

Book Series:

  1. Methods and Principles in Medicinal Chemistry

Book Series Editors:

  1. Prof. Dr. Raimund Mannhold4,
  2. Prof. Dr. Hugo Kubinyi5 and
  3. Prof. Dr. Gerd Folkers6

Series Editor Information

  1. 4

    Molecular Drug Research Group, Heinrich-Heine-Universität, Universitätsstrasse 1, 40225 Düsseldorf, Germany

  2. 5

    Donnersbergerstrasse 9, 67256 Weisenheim am Sand, Germany

  3. 6

    Collegium Helveticum, STW/ETH Zurich, 8092 Zurich, Switzerland

ISBN Information

Print ISBN: 9783527314560

Online ISBN: 9783527610907

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Keywords:

  • library screening;
  • ultrafiltration;
  • mass spectrometry;
  • affinity selection;
  • ultra-high throughput filtration-based affinity screening;
  • promiscuous compound filter;
  • pulsed ultrafiltration MS

Summary

This chapter contains sections titled:

  • Introduction

  • Ultra-high Throughput Filtration-based Affinity Screening as a Discovery Tool

    • Affinity Selection/Mass Spectrometry

    • Primary Screening Strategy

    • Retesting and Deconvolution Strategy

    • Promiscuous Compound Filter

    • MurF Lead Discovery

  • Additional Affinity Screening Methodology that Includes Mass Spectrometry-based Readout

    • Pulsed Ultrafiltration MS

  • Conclusions and Future Directions