Chapter 6. Frontal Affinity Chromatography – Mass Spectrometry for Ligand Discovery and Characterization

  1. Prof. Dr. Klaus T. Wanner and
  2. Dr. Georg Höfner
  1. Nora Chan1,
  2. Darren Lewis2,
  3. Michele Kelly3,
  4. Ella S. M. Ng4 and
  5. David C. Schriemer4

Published Online: 24 MAY 2007

DOI: 10.1002/9783527610907.ch6

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

How to Cite

Chan, N., Lewis, D., Kelly, M., Ng, E. S. M. and Schriemer, D. C. (2007) Frontal Affinity Chromatography – Mass Spectrometry for Ligand Discovery and Characterization, in Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery (eds K. T. Wanner and G. Höfner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527610907.ch6

Editor Information

  1. Ludwig-Maximilians-University Munich, Department of Pharmacy, Center of Drug Research, Butenandtstr., 81377 Munich, Germany

Author Information

  1. 1

    Defence R & D Canada – Suffield, Box 4000, Stn Main, Medicine Hat, Alberta T1A 8K6, Canada

  2. 2

    Upchurch Scientific Inc., 619 Oak Street, Oak Harbor, WA 98277, USA

  3. 3

    Groton/New London Laboratories, Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA

  4. 4

    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada

Publication History

  1. Published Online: 24 MAY 2007
  2. Published Print: 23 FEB 2007

Book Series:

  1. Methods and Principles in Medicinal Chemistry

Book Series Editors:

  1. Prof. Dr. Raimund Mannhold6,
  2. Prof. Dr. Hugo Kubinyi7 and
  3. Prof. Dr. Gerd Folkers8

Series Editor Information

  1. 6

    Molecular Drug Research Group, Heinrich-Heine-Universität, Universitätsstrasse 1, 40225 Düsseldorf, Germany

  2. 7

    Donnersbergerstrasse 9, 67256 Weisenheim am Sand, Germany

  3. 8

    Collegium Helveticum, STW/ETH Zurich, 8092 Zurich, Switzerland

ISBN Information

Print ISBN: 9783527314560

Online ISBN: 9783527610907

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Keywords:

  • frontal affinity chromatography (FAC);
  • mass spectrometry for ligand discovery and characterization;
  • basic frontal method;
  • enabling FAC with MS detection;
  • system advancements;
  • fluidics;
  • immobilization;
  • detection;
  • select applications

Summary

This chapter contains sections titled:

  • Introduction

    • The Basic Frontal Method

    • FAC – Basic Theory

    • FAC Advantages

    • FAC Disadvantages

  • Enabling FAC with MS Detection

    • Direct FAC-MS Methods for Compound Binding Data

    • Direct Method for Discovering and Ranking Multiple Ligands

    • Indirect Methods

  • System Advancements – Fluidics, Immobilization, Detection

    • Column

    • System

    • Breakthrough Curve Detection and Data Analysis

  • Select Applications

  • Summary and Evaluation