Chapter 7. MS Binding Assays – An Alternative to Radioligand Binding

  1. Prof. Dr. Klaus T. Wanner and
  2. Dr. Georg Höfner
  1. Georg Höfner,
  2. Christine Zepperitz and
  3. Klaus T. Wanner

Published Online: 24 MAY 2007

DOI: 10.1002/9783527610907.ch7

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery

How to Cite

Höfner, G., Zepperitz, C. and Wanner, K. T. (2007) MS Binding Assays – An Alternative to Radioligand Binding, in Mass Spectrometry in Medicinal Chemistry: Applications in Drug Discovery (eds K. T. Wanner and G. Höfner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527610907.ch7

Editor Information

  1. Ludwig-Maximilians-University Munich, Department of Pharmacy, Center of Drug Research, Butenandtstr., 81377 Munich, Germany

Author Information

  1. Ludwig-Maximilians-University Munich, Department of Pharmacy, Center of Drug Research, Butenandtstr., 81377 Munich, Germany

Publication History

  1. Published Online: 24 MAY 2007
  2. Published Print: 23 FEB 2007

Book Series:

  1. Methods and Principles in Medicinal Chemistry

Book Series Editors:

  1. Prof. Dr. Raimund Mannhold2,
  2. Prof. Dr. Hugo Kubinyi3 and
  3. Prof. Dr. Gerd Folkers4

Series Editor Information

  1. 2

    Molecular Drug Research Group, Heinrich-Heine-Universität, Universitätsstrasse 1, 40225 Düsseldorf, Germany

  2. 3

    Donnersbergerstrasse 9, 67256 Weisenheim am Sand, Germany

  3. 4

    Collegium Helveticum, STW/ETH Zurich, 8092 Zurich, Switzerland

ISBN Information

Print ISBN: 9783527314560

Online ISBN: 9783527610907

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Keywords:

  • MS binding assays;
  • radioligand binding assays;
  • saturation assays;
  • competition assays;
  • kinetic assays;
  • MS binding assays quantifying the nonbound marker;
  • MS binding assays quantifying the bound marker

Summary

This chapter contains sections titled:

  • Introduction

  • Radioligand Binding Assays

    • General Principle

      • Saturation Assays

      • Competition Assays

      • Kinetic Assays

    • Application

    • Disadvantages and Alternatives

  • MS Binding Assays

    • MS Binding Assays Quantifying the Nonbound Marker

      • Competition Assays for D1 and D2 Dopamine Receptors

      • Library Screening and Competition Assays for μ-Opioid Receptors

    • MS Binding Assays Quantifying the Bound Marker

      • Saturation Assays for mGAT1

      • Competition Assays for mGAT1

      • Kinetic Assays for mGAT1

  • Summary and Perspectives