Chapter 6. Hydrolysis

  1. Prof. Dr. Roger Arthur Sheldon,
  2. Dr. Isabel W. C. E. Arends and
  3. Dr. Ulf Hanefeld

Published Online: 12 APR 2007

DOI: 10.1002/9783527611003.ch6

Green Chemistry and Catalysis

Green Chemistry and Catalysis

How to Cite

Sheldon, R. A., Arends, I. W. C. E. and Hanefeld, U. (2007) Hydrolysis, in Green Chemistry and Catalysis, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527611003.ch6

Author Information

  1. Biocatalysis and Organic Chemistry, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands

Publication History

  1. Published Online: 12 APR 2007
  2. Published Print: 23 FEB 2007

ISBN Information

Print ISBN: 9783527307159

Online ISBN: 9783527611003



  • hydrolysis;
  • stereoselectivity of hydrolases;
  • hydrolase-based preparation of enantiopure compounds;
  • hydrolysis of esters;
  • kinetic resolution of esters;
  • hydrolysis of amides;
  • production of amino acids by (dynamic) kinetic resolution;
  • enzyme-catalysed hydrolysis of amides;
  • hydrolysis of nitriles;
  • nitrilases


This chapter contains sections titled:

  • Introduction

    • Stereoselectivity of Hydrolases

    • Hydrolase-based Preparation of Enantiopure Compounds

      • Kinetic Resolutions

      • Dynamic Kinetic Resolutions

      • Kinetic Resolutions Combined with Inversions

      • Hydrolysis of Symmetric Molecules and the “meso-trick”

  • Hydrolysis of Esters

    • Kinetic Resolutions of Esters

    • Dynamic Kinetic Resolutions of Esters

    • Kinetic Resolutions of Esters Combined with Inversions

    • Hydrolysis of Symmetric Esters and the “meso-trick”

  • Hydrolysis of Amides

    • Production of Amino Acids by (Dynamic) Kinetic Resolution

      • The Acylase Process

      • The Amidase Process

      • The Hydantoinase Process

      • Cysteine

    • Enzyme-catalysed Hydrolysis of Amides

    • Enzyme-catalysed Deprotection of Amines

  • Hydrolysis of Nitriles

    • Nitrilases

    • Nitrile Hydratases

  • Conclusion and Outlook