Chapter 15. Modeling the Blood-Brain Barrier

  1. Prof. Dr. Rolf Dermietzel2,
  2. Prof. Dr. David C. Spray3 and
  3. Prof. Dr. Maiken Nedergaard4
  1. Roméo Cecchelli,
  2. Caroline Coisne,
  3. Lucie Dehouck,
  4. Florence Miller,
  5. Marie-Pierre Dehouck,
  6. Valérie Buée-Scherrer and
  7. Bénédicte Dehouck

Published Online: 31 MAY 2007

DOI: 10.1002/9783527611225.ch15

Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1

Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1

How to Cite

Cecchelli, R., Coisne, C., Dehouck, L., Miller, F., Dehouck, M.-P., Buée-Scherrer, V. and Dehouck, B. (2006) Modeling the Blood-Brain Barrier, in Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1 (eds R. Dermietzel, D. C. Spray and M. Nedergaard), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527611225.ch15

Editor Information

  1. 2

    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany

  2. 3

    Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway S, Bronx, NY 10464, USA

  3. 4

    School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA

Author Information

  1. Faculté des Sciences Jean Perrin, Université d'Artois, SP 18, rue Jean Souvraz, 62307 Lens, France

Publication History

  1. Published Online: 31 MAY 2007
  2. Published Print: 20 FEB 2006

ISBN Information

Print ISBN: 9783527310883

Online ISBN: 9783527611225

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Keywords:

  • culturing blood-brain barrier;
  • modeling blood-brain barrier;
  • culturing brain capillary endothelial cells;
  • in vitro BBB model

Summary

This chapter contains sections titled:

  • Introduction

    • In Vitro BBB Model Interests

    • The BBB: Brain Capillary Endothelial Cells and Brain Parenchyma Cells

  • Culturing Brain Capillary Endothelial Cells

    • Brain Capillary Endothelial Cell Isolation

      • Brain Capillary Endothelial Cell Isolation

      • Endothelial Cell Culture From Capillaries

      • Primary Endothelial Cells and Subculture of Brain Capillary Endothelial Cells

      • Immortalization

      • Purity

      • Species

    • Coculture

  • Characteristics Required for a Useful In Vitro BBB Model

    • Confluent Monolayer

    • Tight Junctions and Paracellular Permeability

    • Transcellular Transport, Receptor Mediated Transport

    • Expression of Endothelial Adhesion Molecules/Vascular Inflammatory Markers

  • Conclusion

  • References