Chapter 16. Induction of Blood-Brain Barrier Properties in Cultured Endothelial Cells

  1. Prof. Dr. Rolf Dermietzel4,
  2. Prof. Dr. David C. Spray5 and
  3. Prof. Dr. Maiken Nedergaard6
  1. Alla Zozulya1,
  2. Christian Weidenfeller2 and
  3. Hans-Joachim Galla3

Published Online: 31 MAY 2007

DOI: 10.1002/9783527611225.ch16

Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1

Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1

How to Cite

Zozulya, A., Weidenfeller, C. and Galla, H.-J. (2006) Induction of Blood-Brain Barrier Properties in Cultured Endothelial Cells, in Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1 (eds R. Dermietzel, D. C. Spray and M. Nedergaard), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527611225.ch16

Editor Information

  1. 4

    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany

  2. 5

    Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway S, Bronx, NY 10464, USA

  3. 6

    School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA

Author Information

  1. 1

    Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53706, USA

  2. 2

    Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA

  3. 3

    Department of Biochemistry, University of Münster, Wilhelm-Klemm-Strasse 2, 48149 Münster, Germany

Publication History

  1. Published Online: 31 MAY 2007
  2. Published Print: 20 FEB 2006

ISBN Information

Print ISBN: 9783527310883

Online ISBN: 9783527611225

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Keywords:

  • culturing blood-brain barrier;
  • induction of blood-brain barrier properties;
  • cultured endothelial cells;
  • in vitro BBB models;
  • hydrocortisone reinforces barrier properties;
  • primary cultured cerebral endothelial cells;
  • serum effects;
  • hydrocortisone improves culture substrate;
  • suppressing expression of matrix metalloproteinases in vitro;
  • role of endogenously derived ECM for BBB properties

Summary

This chapter contains sections titled:

  • Introduction

  • In Vitro BBB Models

  • Hydrocortisone Reinforces the Barrier Properties of Primary Cultured Cerebral Endothelial Cells

    • In Vitro Model Based on Pig Brain Capillary Endothelial Cells (PBCEC)

    • In Vitro Model Based on Mouse Brain Capillary Endothelial Cells (MBCEC)

  • The Involvement of Serum Effects

  • Hydrocortisone Improves the Culture Substrate by Suppressing the Expression of Matrix Metalloproteinases In Vitro

    • ECIS Analysis of Improved Endothelial Cell-Cell and Cell-Substrate Contacts in HC-Supplemented Medium

    • Low Degradation of ECM in HC-Supplemented Medium Leads to Improved Cell-Substrate Contacts of Cerebral Endothelial Cells

  • The Role of Endogenously Derived ECM for the BBB Properties of Cerebral Endothelial Cells In Vitro

  • Conclusions

  • References