Chapter 17. Artificial Blood-Brain Barriers

  1. Prof. Dr. Rolf Dermietzel3,
  2. Prof. Dr. David C. Spray4 and
  3. Prof. Dr. Maiken Nedergaard5
  1. Luca Cucullo1,
  2. Emily Oby1,
  3. Kerri Hallene1,
  4. Barbara Aumayr1,
  5. Ed Rapp2 and
  6. Damir Janigro1

Published Online: 31 MAY 2007

DOI: 10.1002/9783527611225.ch17

Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1

Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1

How to Cite

Cucullo, L., Oby, E., Hallene, K., Aumayr, B., Rapp, E. and Janigro, D. (2006) Artificial Blood-Brain Barriers, in Blood-Brain Barriers: From Ontogeny to Artificial Interfaces, Volume 1 (eds R. Dermietzel, D. C. Spray and M. Nedergaard), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527611225.ch17

Editor Information

  1. 3

    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany

  2. 4

    Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway S, Bronx, NY 10464, USA

  3. 5

    School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA

Author Information

  1. 1

    Department of Neurosurgery, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44106, USA

  2. 2

    Cleveland Medical Devices Inc., 4415 Euclid Avenue, Cleveland, OH 44103, USA

Publication History

  1. Published Online: 31 MAY 2007
  2. Published Print: 20 FEB 2006

ISBN Information

Print ISBN: 9783527310883

Online ISBN: 9783527611225



  • culturing blood-brain barrier;
  • artificial blood-brain barriers;
  • requirements for a good BBB model;
  • immobilized artificial membranes;
  • cell culture-based in vitro BBB models;
  • shear stress;
  • cell differentiation;
  • flow-based in vitro BBB systems


This chapter contains sections titled:

  • Introduction: The Blood-Brain Barrier

  • Requirements for a Good BBB Model

  • Immobilized Artificial Membranes

  • Cell Culture-Based in vitro BBB Models

    • Cell Lines

      • Immortalized Rat Brain Endothelial Cells

      • Other Cells From Non-Cerebral Sources

      • Brain Capillary Endothelial Cell Cultures

    • Monoculture-Based in vitro BBB Models

    • Coculture-Based in vitro BBB Models

  • Shear Stress and Cell Differentiation

  • Flow-Based in vitro BBB Systems

    • Dynamic in vitro BBB: Standard Model

    • Dynamic in vitro BBB: New Model

  • A Look Into The Future: Automated Flow Based in vitro BBBs

  • Conclusion

  • References