Chapter 2. Cholera Toxin and Escherichia coli Heat-Labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities

  1. Klaus Aktories
  1. W. A. Patton,
  2. G.-F. Zhang,
  3. J. Moss,
  4. M. Vaughan

Published Online: 5 JUN 2008

DOI: 10.1002/9783527614615.ch2

Book Title

Bacterial Toxins: Tools in Cell Biology and Pharmacology

Bacterial Toxins: Tools in Cell Biology and Pharmacology

Additional Information

How to Cite

Patton, W. A., Zhang, G.-F., Moss, J. and Vaughan, M. (2008) Cholera Toxin and Escherichia coli Heat-Labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities, in Bacterial Toxins: Tools in Cell Biology and Pharmacology (ed K. Aktories), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527614615.ch2

Publication History

  1. Published Online: 5 JUN 2008
  2. Published Print: 24 APR 1997

ISBN Information

Print ISBN: 9783527308811

Online ISBN: 9783527614615

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Keywords:

  • enzymatically;
  • nicotinamide;
  • heterotrimeric;
  • eukaryotic;
  • phosphocellulose

Summary

The prelims comprise:

  • Introduction

  • General Information on CT, LT, ARF and Reagents

  • Assay 1 : The Gsα, Assay

  • Assay 2: The Agmatine Assay

  • Assay 3: Auto-ADP-ribosylation Assay

  • Assay 4: NAD Glycohydrolase Assay

  • Comments and Considerations

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