Chapter 6. Clostridium botulinum C3 Exoenzyme and Studies on Rho Proteins

  1. Klaus Aktories
  1. C. D. Nobes and
  2. A. Hall

Published Online: 5 JUN 2008

DOI: 10.1002/9783527614615.ch6

Bacterial Toxins: Tools in Cell Biology and Pharmacology

Bacterial Toxins: Tools in Cell Biology and Pharmacology

How to Cite

Nobes, C. D. and Hall, A. (1997) Clostridium botulinum C3 Exoenzyme and Studies on Rho Proteins, in Bacterial Toxins: Tools in Cell Biology and Pharmacology (ed K. Aktories), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527614615.ch6

Publication History

  1. Published Online: 5 JUN 2008
  2. Published Print: 24 APR 1997

ISBN Information

Print ISBN: 9783527308811

Online ISBN: 9783527614615

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Keywords:

  • glutathione S-transferase;
  • polylinker;
  • phenylmethylsulfonyl fluoride;
  • microinjection;
  • gel electrophoresis

Summary

The prelims comprise:

  • Introduction

  • Purification of Recombinant C3 Transferase from Escherichia coli

  • Preparation of Swiss 3T3 Cells

  • Microinjection of C3 Transferase

  • Fixation and Staining of Cells

  • C3 Protein Inhibits LPA-stimulated Stress Fibre Assembly and Focal Adhesion Clustering

  • Timecourse of Focal Adhesion Breakdown

  • Alternative Delivery of C3 Transferase

  • Conclusion

  • Reagents and Chemicals