Chapter 6. Clostridium botulinum C3 Exoenzyme and Studies on Rho Proteins
- Klaus Aktories
Published Online: 5 JUN 2008
DOI: 10.1002/9783527614615.ch6
Copyright © 2002 WILEY-VCH Verlag GmbH & Co. KGaA
Book Title
Bacterial Toxins: Tools in Cell Biology and Pharmacology
Additional Information
How to Cite
Nobes, C. D. and Hall, A. (2008) Clostridium botulinum C3 Exoenzyme and Studies on Rho Proteins, in Bacterial Toxins: Tools in Cell Biology and Pharmacology (ed K. Aktories), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527614615.ch6
Publication History
- Published Online: 5 JUN 2008
- Published Print: 24 APR 1997
ISBN Information
Print ISBN: 9783527308811
Online ISBN: 9783527614615
- Summary
- Chapter
- References
Keywords:
- glutathione S-transferase;
- polylinker;
- phenylmethylsulfonyl fluoride;
- microinjection;
- gel electrophoresis
Summary
The prelims comprise:
Introduction
Purification of Recombinant C3 Transferase from Escherichia coli
Preparation of Swiss 3T3 Cells
Microinjection of C3 Transferase
Fixation and Staining of Cells
C3 Protein Inhibits LPA-stimulated Stress Fibre Assembly and Focal Adhesion Clustering
Timecourse of Focal Adhesion Breakdown
Alternative Delivery of C3 Transferase
Conclusion
Reagents and Chemicals