Chapter 10. The SIAT Research, Teaching and Consulting Program in the Area of in Vitro Toxicology. Experimental Research, Screening and Validation

  1. Dr. Christoph A Reinhardt
  1. Christoph A. Reinhardt

Published Online: 8 OCT 2008

DOI: 10.1002/9783527616053.ch10

Alternatives to Animal Testing: New Ways in the Biomedical Sciences, Trends and Progress, Second Edition

Alternatives to Animal Testing: New Ways in the Biomedical Sciences, Trends and Progress, Second Edition

How to Cite

Reinhardt, C. A. (2008) The SIAT Research, Teaching and Consulting Program in the Area of in Vitro Toxicology. Experimental Research, Screening and Validation, in Alternatives to Animal Testing: New Ways in the Biomedical Sciences, Trends and Progress, Second Edition (ed C. A. Reinhardt), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527616053.ch10

Editor Information

  1. SIAT Swiss Institute for Alternatives to Animal Testing, Technopark, Pfingstweidstr. 30, CH-8005 Zürich

Publication History

  1. Published Online: 8 OCT 2008
  2. Published Print: 24 FEB 1994

ISBN Information

Print ISBN: 9783527300433

Online ISBN: 9783527616053

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Keywords:

  • vitro Toxicology;
  • alternative techniques;
  • SIAT;
  • functional neural cells;
  • cultured reaggregates

Summary

The objectives and activities of the Swiss Institute for Alternatives to Animal Testing, SIAT, are focused on research, education, and providing consultation in the field of alternatives to animal testing according to the Three Rs (Refine, Reduce, Replace). Practical courses, workshops and lectures are offered at the university level, in cooperation with the Swiss Federal Institute of Technology (ETH) and the University of Zurich. Documentation and consulting is provided in the areas of toxicology, biotechnology, human and veterinary medicine, ecology and bioethics.

The In Vitro Toxicology Group has put its main emphasis on neurodevelopmental research. Brain and retinal cells from chick embryos and from adult bovine brain are used as “robust” in vitro models including a blood-brain-barrier system for teratology and neurotoxi-cology. Morphology, cytotoxicity and differentiation markers of nerve, glial and endothelial cells are monitored to predict cell toxicity of drugs and chemicals. A new screening system “CHEN” (chick embryo neural cell system) is presented, which is recommended for an early screening phase in product development covering neuroteratological adverse effects. CHEN, combined with the blood-brain-barrier model and other in vitro assays, can lead to a substantial reduction of whole animal testing in screening and risk assessment.