Chapter 2. Membrane-Bound Enzymes

  1. Prof. Dr. Meir Shinitzky
  1. G. Lenaz1 and
  2. M. Degli Esposti2

Published Online: 4 APR 2008

DOI: 10.1002/9783527616114.ch2

Biomembranes: Structural and Functional Aspects

Biomembranes: Structural and Functional Aspects

How to Cite

Lenaz, G. and Esposti, M. D. (1994) Membrane-Bound Enzymes, in Biomembranes: Structural and Functional Aspects (ed M. Shinitzky), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527616114.ch2

Editor Information

  1. Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot 76120, Israel

Author Information

  1. 1

    Department of Biochemistry, University of Bologna, Bologna, Italy

  2. 2

    Department of Biology, University of Bologna, Bologna, Italy

Publication History

  1. Published Online: 4 APR 2008
  2. Published Print: 27 JAN 1994

ISBN Information

Print ISBN: 9783527300228

Online ISBN: 9783527616114

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Keywords:

  • anchored enzymes;
  • fatty acylation;
  • compartmentalization;
  • protein mobility;
  • proton translocation

Summary

The prelims comprise:

  • Abbreviations

  • Introduction

  • Molecular Structure of Membrane Bound Enzymes

  • Sidedness and Topography of Membrane Enzymes

  • Isolation and Reconstitution of Membrane Enzymes

  • Consequences of Membrane Binding

  • Role of Lipids in Membrane Bound Enzymes

  • Determination of the Kinetic Constants of Enzymes with Hydrophobic Substrates

  • Kinetics of Membrane Bound Versus Soluble Enzymes

  • Evidence for Conformational Changes of Membrane-Bound Enzymes upon Lipid Changes

  • Acknowledgements

  • References