Chapter 19. Energy-Variable X-Ray Diffraction with High Depth Resolution Used for Mollusk Shell Analysis

  1. Prof. Dr. Edmund Bäuerlein
  1. Emil Zolotoyabko

Published Online: 20 MAR 2008

DOI: 10.1002/9783527619443.ch43

Handbook of Biomineralization: Biological Aspects and Structure Formation

Handbook of Biomineralization: Biological Aspects and Structure Formation

How to Cite

Zolotoyabko, E. (2007) Energy-Variable X-Ray Diffraction with High Depth Resolution Used for Mollusk Shell Analysis, in Handbook of Biomineralization: Biological Aspects and Structure Formation (ed E. Bäuerlein), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527619443.ch43

Editor Information

  1. Max-Planck-Institute for Biochemistry, Department of Membrane Biochemistry, Am Klopferspitz 18 A, 82152 Planegg, Germany

Publication History

  1. Published Online: 20 MAR 2008
  2. Published Print: 25 MAY 2007

ISBN Information

Print ISBN: 9783527316410

Online ISBN: 9783527619443

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Keywords:

  • biomineralization;
  • mollusk shells;
  • microstructure;
  • x-ray diffraction;
  • synchrotron radiation;
  • grain size;
  • residual strains;
  • microstrain fluctuations;
  • preferred orientation

Summary

Recently, we have developed a novel X-ray diffraction (XRD) technique which allows us to obtain structural and microstructural characteristics in polycrystalline materials with high depth resolution. The method is based on the analysis of the XRD peaks collected at various X-ray energies, the latter being controllably changed by sufficiently small steps. By increasing energy and, correspondingly, the X-ray penetration length, the progressively deeper material's layers are probed. A theoretical analysis of the problem showed that the depth resolution is determined primarily by the energy steps applied and, thus, the sub-micrometer range for the depth steps is easily achievable. Application of this technique to some aragonitic mollusk shells resulted in the depth-resolved microstructural characteristics, such as preferred orientation, lamella size, averaged microstrain fluctuations and residual strains, which previously were not accessible.