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Chapter 25. UV Melting, Native Gels and RNA Conformation

  1. Prof. Dr. Roland K. Hartmann1,
  2. Prof. Dr. Albrecht Bindereif2,
  3. Dr. Astrid Schön3,
  4. Prof. Dr. Eric Westhof4
  1. Andreas Werner

Published Online: 5 FEB 2008

DOI: 10.1002/9783527619504.ch25

Handbook of RNA Biochemistry

Handbook of RNA Biochemistry

How to Cite

Werner, A. (2008) UV Melting, Native Gels and RNA Conformation, in Handbook of RNA Biochemistry (eds R. K. Hartmann, A. Bindereif, A. Schön and E. Westhof), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527619504.ch25

Editor Information

  1. 1

    Philipps-Universität Marburg, Institut für Pharmazeutische Chemie, Marbacher Weg 6, 35037 Marburg, Germany roland.hartmann@sta.uni-marburg.de

  2. 2

    Justus-Liebig-Universität Giessen, Institut für Biochemie, Heinrich-Buff-Ring 58, 35392 Giessen, Germany, albrecht.bindereif@chemie.bio.uni-giessen.de

  3. 3

    Universität Leipzig, Institut für Biochemie, Brüderstr. 34, 04103 Leipzig, Germany schoena@uni-leipzig.de

  4. 4

    CNRS-UPR 9002, Institut de Biologie Moléculaire et, Cellulaire, 15 rue René Descartes, 67084 Strasbourg, France, e.westhof@ibmc.u-strasbg.fr

Publication History

  1. Published Online: 5 FEB 2008
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527308262

Online ISBN: 9783527619504

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Keywords:

  • conformation;
  • native gels;
  • equilibrium;
  • spectrophotometer;
  • cuvettes

Summary

This chapter contains sections titled:

  • Monitoring RNA Folding in Solution

  • Methods

  • Data Analysis

  • Energy Calculations and Limitations

  • RNA Concentration

  • Salt and pH Dependence

  • Native Gels