20. In vitro Protein Refolding

  1. H.-J. Rehm3,
  2. G. Reed4
  1. Jeffrey L. Cleland1,
  2. Daniel I. C. Wang2

Published Online: 26 MAY 2008

DOI: 10.1002/9783527620845.ch20

Biotechnology: Bioprocessing, Volume 3, Second Edition

Biotechnology: Bioprocessing, Volume 3, Second Edition

How to Cite

Cleland, J. L. and Wang, D. I. C. (2008) In vitro Protein Refolding, in Biotechnology: Bioprocessing, Volume 3, Second Edition (eds H.-J. Rehm and G. Reed), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527620845.ch20

Editor Information

  1. 3

    Institut für Mikrobiologie, Universität, Münster, Corrensstraße 3, D-48149 Münster

  2. 4

    2131 N. Summit Ave., Apartment #304, Milwaukee, WI 53202-1347, USA

Author Information

  1. 1

    South San Francisco, CA 94080, USA

  2. 2

    Cambridge, MA 02139, USA

Publication History

  1. Published Online: 26 MAY 2008
  2. Published Print: 28 OCT 1993

ISBN Information

Print ISBN: 9783527283132

Online ISBN: 9783527620845

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Keywords:

  • biophysical;
  • pharmaceutical;
  • variations;
  • proportion;
  • cysteine

Summary

This chapter contains sections titled:

  • Introduction

  • Folding in Eukaryotic and Prokaryotic Hosts

  • Inclusion Body Formation

  • Effect of Folding Mutations

  • Molecular Chaperones

  • Synthetic Folding Aids

  • Models of in vitro Protein Refolding

  • In vitro Refolding Dependence on Denaturant Concentration

  • Temperature Effects on Refolding

  • Aggregation During Refolding

  • Summary

  • References