Chapter 23. Protein Separation and Purification

  1. Prof. Dr. H.-J. Rehm2 and
  2. Dr. G. Reed3
  1. Jan-Christer Janson and
  2. Lars Rydén

Published Online: 7 MAY 2008

DOI: 10.1002/9783527620999.ch23b

Biotechnology Set, Second Edition

Biotechnology Set, Second Edition

How to Cite

Janson, J.-C. and Rydén, L. (2001) Protein Separation and Purification, in Biotechnology Set, Second Edition (eds H.-J. Rehm and G. Reed), Wiley-VCH Verlag GmbH, Weinheim, Germany. doi: 10.1002/9783527620999.ch23b

Editor Information

  1. 2

    Institut für Mikrobiologie, Universität Münster, Corrensstraße 3, D-4400 Münster

  2. 3

    2131 N. Summit Ave., Apartment #304, Milwaukee, WI 53202-1347, USA

Author Information

  1. Uppsala, Sweden

Publication History

  1. Published Online: 7 MAY 2008
  2. Published Print: 10 MAY 2001

ISBN Information

Print ISBN: 9783527257621

Online ISBN: 9783527620999

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Keywords:

  • evolution;
  • antibody;
  • pharmaceutical;
  • impurities;
  • molecular biology

Summary

This chapter contains sections titled:

  • Introduction - From Art to Rational Protein Management

  • Strategies for Protein Separation

  • Techniques for Initial Fractionation of Crude Extracts

  • Protein Chromatography - General Aspects

  • Protein Size Methods - Gel Filtration

  • Protein Charge Methods - Ion Exchange Chromatography and Chromatofocusing

  • Protein Hydrophobicity Methods - Hydrophobic Interaction Chromatography and Protein Affinity Methods - Affinity Chromatography

  • Concluding Remarks

  • Reversed Phase Chromatography

  • References