Chapter 11. Membrane Protein Structure and Conformational Change Probed using Fourier Transform Infrared Spectroscopy

  1. Prof. Eva Pebay-Peyroula PhD professor
  1. John E. Baenziger and
  2. Corrie J. B. daCosta

Published Online: 7 JAN 2008

DOI: 10.1002/9783527621224.ch11

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

How to Cite

Baenziger, J. E. and daCosta, C. J. B. (2007) Membrane Protein Structure and Conformational Change Probed using Fourier Transform Infrared Spectroscopy, in Biophysical Analysis of Membrane Proteins: Investigating Structure and Function (ed E. Pebay-Peyroula), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527621224.ch11

Editor Information

  1. Institut de Biologie Structurale Jean-Pierre Ebel, Université Joseph Fourier-CEA-CNRS, 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France

Author Information

  1. Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada

Publication History

  1. Published Online: 7 JAN 2008
  2. Published Print: 24 OCT 2007

ISBN Information

Print ISBN: 9783527316779

Online ISBN: 9783527621224

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Keywords:

  • biophysical analysis;
  • spectroscopies;
  • membrane protein structure;
  • conformational change;
  • Fourier transform infrared spectroscopy (FTIR Spectroscopy);
  • vibrational spectra;
  • applications

Summary

This chapter contains sections titled:

  • Introduction

  • FTIR Spectroscopy

    • Attenuated Total Reflectance FTIR Spectroscopy

    • Detecting Changes in Side Chain Structure/Environment During Protein Conformational Change

    • Probing the Orientation of Functional Groups

  • Vibrational Spectra of Membrane Proteins

    • Lipid Vibrations

      • Lipid Ester C[DOUBLE BOND]O

      • Lipid Methylene C[BOND]H

    • Protein Backbone Vibrations

      • Amide I

      • Amide II

    • Protein Side-Chain Vibrations

  • Applications of FTIR To Membrane Proteins

    • Testing Protein Structural Models and Validating the Structures of Mutant Proteins

    • Lipid–Protein Interactions

    • Receptor–Drug Interactions

    • Chemistry of Receptor–Ligand Interactions

    • Changes in Orientation of Functional Groups During Conformational Change

    • A Tool in the Crystallization of Integral Membrane Proteins

  • Conclusions and Future Directions