Chapter 2. Membrane Protein Structure Determination by Electron Cryo-Microscopy

  1. Prof. Eva Pebay-Peyroula PhD professor
  1. Christopher G. Tate1 and
  2. John L. Rubinstein2

Published Online: 7 JAN 2008

DOI: 10.1002/9783527621224.ch2

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

How to Cite

Tate, C. G. and Rubinstein, J. L. (2007) Membrane Protein Structure Determination by Electron Cryo-Microscopy, in Biophysical Analysis of Membrane Proteins: Investigating Structure and Function (ed E. Pebay-Peyroula), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527621224.ch2

Editor Information

  1. Institut de Biologie Structurale Jean-Pierre Ebel, Université Joseph Fourier-CEA-CNRS, 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France

Author Information

  1. 1

    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom

  2. 2

    Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto M5G 1X8, Canada

Publication History

  1. Published Online: 7 JAN 2008
  2. Published Print: 24 OCT 2007

ISBN Information

Print ISBN: 9783527316779

Online ISBN: 9783527621224

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Keywords:

  • biophysical analysis;
  • membrane proteins;
  • structural approaches;
  • structure determination;
  • electron cryo-microscopy;
  • single-particle electron microscopy;
  • 2-dimensional crystals;
  • helical analysis of tubes

Summary

This chapter contains sections titled:

  • Introduction

    • The Electron Microscope

  • Single-Particle Electron Microscopy

    • Sample Preparation and Requirements

      • Negative Staining of Specimens

      • Cryo-EM of Unstained Specimens

      • Choice of detergent

    • Image Analysis

      • Classification of Images

      • Model Building and Refinement

      • Assessing Resolution

    • Future Perspectives

  • Structure Determination from 2-Dimensional Crystals

    • Two-Dimensional Crystallization of Membrane Proteins

    • Image Acquisition and Structure Determination

    • Future Perspectives

  • Helical Analysis of Tubes

  • Conclusions