Chapter 3. Introduction to Solid-State NMR and its Application to Membrane Protein–Ligand Binding Studies

  1. Prof. Eva Pebay-Peyroula PhD professor
  1. Krisztina Varga and
  2. Anthony Watts

Published Online: 7 JAN 2008

DOI: 10.1002/9783527621224.ch3

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

How to Cite

Varga, K. and Watts, A. (2007) Introduction to Solid-State NMR and its Application to Membrane Protein–Ligand Binding Studies, in Biophysical Analysis of Membrane Proteins: Investigating Structure and Function (ed E. Pebay-Peyroula), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527621224.ch3

Editor Information

  1. Institut de Biologie Structurale Jean-Pierre Ebel, Université Joseph Fourier-CEA-CNRS, 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France

Author Information

  1. University of Oxford, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, United Kingdom

Publication History

  1. Published Online: 7 JAN 2008
  2. Published Print: 24 OCT 2007

ISBN Information

Print ISBN: 9783527316779

Online ISBN: 9783527621224

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Keywords:

  • biophysical analysis;
  • membrane proteins;
  • structural approaches;
  • solid-state NMR;
  • application;
  • membrane protein–ligand binding studies;
  • receptor–ligand studies;
  • transport proteins

Summary

This chapter contains sections titled:

  • Introduction

    • Membrane Proteins: A Challenge

    • Why Solid-State NMR?

  • Solid-State NMR

    • Sample Preparation: What is an Ideal Sample?

      • Availability

      • Stability

      • Secondary Structure

      • Sample Form: Local Order

    • NMR Active Isotopes and Labeling

    • Assignment and Structure Determination

    • NMR Techniques: Solution- versus Solid-State NMR

      • Isotropic Liquids

      • Anisotropic Liquids

      • Solids

  • Examples: Receptor–Ligand Studies by Solid-State NMR

    • Transport Proteins

      • LacS

    • G-Protein-Coupled Receptors and Related Proteins

      • Bacteriorhodopsin, Rhodopsin, and Sensory Rhodopsin (NpSRII)

      • Human H1 Receptor

      • Neurotensin Receptor

    • Ion Channels

      • Nicotinic Acetylcholine Receptor

      • K+ Ion Channel, KcsA

    • P-type ATPases

    • Membrane Protein Soluble Alternatives