Chapter 5. Probing Membrane Protein Interactions with Real-Time Biosensor Technology

  1. Prof. Eva Pebay-Peyroula PhD professor
  1. Iva Navratilova,
  2. David G. Myszka and
  3. Rebecca L. Rich

Published Online: 7 JAN 2008

DOI: 10.1002/9783527621224.ch5

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

How to Cite

Navratilova, I., Myszka, D. G. and Rich, R. L. (2007) Probing Membrane Protein Interactions with Real-Time Biosensor Technology, in Biophysical Analysis of Membrane Proteins: Investigating Structure and Function (ed E. Pebay-Peyroula), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527621224.ch5

Editor Information

  1. Institut de Biologie Structurale Jean-Pierre Ebel, Université Joseph Fourier-CEA-CNRS, 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France

Author Information

  1. Center for Biomolecular Interaction Analysis, School of Medicine 4A417, University of Utah, Salt Lake City, Utah 84132, USA

Publication History

  1. Published Online: 7 JAN 2008
  2. Published Print: 24 OCT 2007

ISBN Information

Print ISBN: 9783527316779

Online ISBN: 9783527621224

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Keywords:

  • biophysical analysis;
  • membrane proteins;
  • molecular interaction;
  • large assemblies;
  • membrane protein interactions;
  • real-time biosensor technology;
  • interactions;
  • extracellular domains;
  • soluble proteins with lipid layers;
  • proteins embedded in lipid layers

Summary

This chapter contains sections titled:

  • Introduction

  • Interactions of Extracellular Domains

  • Interactions of Soluble Proteins with Lipid Layers

  • Interactions of Proteins Embedded in Lipid Layers

    • On-Surface Reconstitution of G-Protein-Coupled Receptor

    • Capture/Reconstitution of GPCRs

  • Interactions of Membrane-Solubilized Proteins

  • Summary