Chapter 2. Diagnostic Immunohistochemistry

  1. Dr. Muin S. A. Tuffaha

Published Online: 14 FEB 2008

DOI: 10.1002/9783527621521.ch2

Book Title

Phenotypic and Genotypic Diagnosis of Malignancies: An Immunohistochemical and Molecular Approach

Phenotypic and Genotypic Diagnosis of Malignancies: An Immunohistochemical and Molecular Approach

Additional Information

How to Cite

Tuffaha, M. S. A. (2008) Diagnostic Immunohistochemistry, in Phenotypic and Genotypic Diagnosis of Malignancies: An Immunohistochemical and Molecular Approach, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527621521.ch2

Author Information

  1. German Jordan Center for Laboratory Medicine, P.O. Box 925705, Amman, 11190, Jordan

Publication History

  1. Published Online: 14 FEB 2008
  2. Published Print: 12 MAR 2008

ISBN Information

Print ISBN: 9783527318810

Online ISBN: 9783527621521

SEARCH

CHAPTER TOOLS

Get PDF (283K)

Keywords:

  • malignancies;
  • phenotypic diagnosis;
  • genotypic diagnosis;
  • diagnostic immunohistochemistry;
  • pharmacopathology;
  • steroid hormone receptor status evaluation;
  • semi-quantitative estimation of receptor expression;
  • immunophenotypic profiles of tumors;
  • antibodies for tumor diagnosis;
  • paraffin sections;
  • expression pattern;
  • immunohistochemical staining;
  • avidin–biotin horseradish detection system

Summary

This chapter contains sections titled:

  • Introduction

  • Pharmacopathology

    • Evaluation of Steroid Hormone Receptor Status by Semi-Quantitative Estimation of Receptor Expression (Estrogen, Progesterone and Androgen Receptors)

      • Estrogen Receptor

      • Progesterone Receptors

      • Androgen Receptors

    • Estimation of Human Epidermal Growth Factor Receptor-2 Expression

    • Estimation of Epidermal Growth Factor Receptor Expression

    • Detection of CD 20

    • Detection of C-Kit Oncoprotein (CD117)

  • Immunophenotypic Profiles of Tumors

    • Soft Tissue Tumors

      • Fibrous and Myofibroblastic Tumors

      • Fibrohistiocytic Tumors

      • Lipomatous Tumors

      • Smooth Muscle Tumors

      • Skeletal Muscle Tumors

      • Endothelial Tumors

      • Perivascular Tumors

      • Peripheral Nerve Tumors

      • Primitive Neuroectodermal Tumors and Related Lesions

      • Mesothelial Tumors

      • Synovial Tumors

      • Extraskeletal Osseous and Cartilaginous Tumors

      • Melanocytic Tumors

      • Miscellaneous Tumors and Tumors of Uncertain Differentiation

    • Central Nervous System Tumors

    • Respiratory Tract and Lung Tumors

      • Upper Respiratory Tract Tumors

      • Lung Tumors

    • Pituitary Gland Tumors

    • Thyroid Tumors

    • Parathyroid Tumors

    • Thymic Epithelial Tumors

    • Salivary Gland Tumors and Odontogenic Tumors

      • Salivary Gland Tumors

      • Odontogenic Tumors

    • Gastrointestinal Tract Tumors

      • Esophageal and Gastric Tumors

      • Intestinal Tumors

      • Gastrointestinal Neuroendocrine Tumors

      • Gastrointestinal Stromal Tumors

    • Hepatobiliary Tumors

    • Pancreas Tumors

      • Tumors of Exocrine Pancreas

      • Tumors of Endocrine Pancreas

    • Adrenal Gland Tumors

    • Renal, Urinary Tract and Urinary Bladder Tumors

      • Renal Tumors

      • Urinary Tract and Urinary Bladder Tumors

    • Breast Tumors

    • Female Genital Tract Tumors

      • Tumors of Uterine Cervix and Uterine Corpus

      • Ovarian Tumors

    • Prostate and Seminal Vesicle Tumors

    • Testicular and Paratesticular Tumors

      • Testicular Tumors

      • Paratesticular Tumors

    • Lymphoid Neoplasia

      • Evolution of Immunophenotypic Profile of Non-Neoplastic Lymphocytes

      • B-Cell Neoplasia

      • T-Cell Neoplasia

      • Hodgkin's Lymphoma

    • Myeloid Neoplasia

      • Evolution of Immunophenotypic Profile of Non-Neoplastic Myeloid Cells

      • Acute Myeloid Leukemia

      • Chronic Myeloid Neoplasia

    • Histiocytic and Dendritic Cell Tumors

  • Common Antibodies used for Tumor Diagnosis on Paraffin Sections and Expression Pattern

  • Immunohistochemical Staining: Avidin–Biotin Horseradish Detection System

    • Standard Staining Protocol (ABC Method)

    • Troubleshooting and Problems Occurring During Immunohistochemical Staining

      • Overstaining

      • Non-Specific Background Staining

      • Weak Staining

      • No Staining

    • Preparing of Solutions and Slides for Immunohistochemical Staining

      • Buffered Neutral Formalin (10%)

      • Phosphate Buffer Saline 0.01 M

      • Citrate Buffer, pH 6 (10 × Stock Solution)

      • EDTA Buffer, pH 8 (1 mM Working Solution)

      • Tris/EDTA/Tween Buffer, pH 9 (10 × Stock Solution)

      • EDTA Decalcification Solution

      • DAB Stock Solution

      • Coated Slides for Immunohistochemical Staining

Get PDF (283K)