Chapter 9. Pseudomonas aeruginosa: A Model for Biofilm Formation

  1. Prof. Dr. Bernd H. A. Rehm
  1. Diane McDougald1,
  2. Janosch Klebensberger1,
  3. Tim Tolker-Nielsen2,
  4. Jeremy S. Webb3,
  5. Tim Conibear4,
  6. Scott A. Rice1,
  7. Sylvia M. Kirov5,
  8. Carsten Matz6 and
  9. Staffan Kjelleberg3

Published Online: 31 MAR 2008

DOI: 10.1002/9783527622009.ch9

Pseudomonas: Model Organism, Pathogen, Cell Factory

Pseudomonas: Model Organism, Pathogen, Cell Factory

How to Cite

McDougald, D., Klebensberger, J., Tolker-Nielsen, T., Webb, J. S., Conibear, T., Rice, S. A., Kirov, S. M., Matz, C. and Kjelleberg, S. (2008) Pseudomonas aeruginosa: A Model for Biofilm Formation, in Pseudomonas: Model Organism, Pathogen, Cell Factory (ed B. H. A. Rehm), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527622009.ch9

Editor Information

  1. Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand

Author Information

  1. 1

    University of New South Wales, School of Biotechnology and Biomolecular Science and Center for Marine Biofouling and Bio-Innovation, Sydney, NSW 2052, Australia

  2. 2

    Technical University of Denmark, Center for Biomedical Microbiology, 2800 Lyngby, Denmark

  3. 3

    University of New South Wales, School of Biotechnology and Biomolecular Sciences, Sydney NSW 2052, Australia

  4. 4

    University of Southampton, School of Biological Sciences, Southampton SO17 1BJ, UK

  5. 5

    University of Tasmania, School of Medicine (Pathology), Hobart, Tasmania 7001, Australia

  6. 6

    Helmhotz Center for Infection Research, Division of Microbiology, 38124 Braunschweig, Germany

Publication History

  1. Published Online: 31 MAR 2008
  2. Published Print: 26 MAR 2008

ISBN Information

Print ISBN: 9783527319145

Online ISBN: 9783527622009



  • Pseudomonas aeruginosa;
  • model for biofilm formation;
  • biofilm development;
  • regulatory mechanisms;
  • c-di-GMP;
  • biofilm/eukaryote interactions


This chapter contains sections titled:

  • Introduction

  • Biofilm Development

    • Early Attachment and Colonization Events

      • Translocation and Attachment to Surfaces

      • Role of Flagella and Fimbriae in Reversible and Irreversible Attachment

    • Extracellular Matrix

      • Polysaccharide

      • Protein

      • DNA

    • Phenotypic and Genotypic Diversification in Pseudomonas Biofilms

      • Ecological and Genetic Causes of Diversity in Pseudomonas Biofilms

      • Colony Morphology Variation in P. aeruginosa Biofilms

      • GacS Sensor Kinase

      • Bacteriophage and SCVs

    • Biofilm Dispersal

      • Role of c-di-GMP in Dispersal

      • Denitrification, Nitric Oxide and Dispersal in P. aeruginosa

      • Microcolony-based Biofilm Dispersal

  • Regulatory Mechanisms of P. aeruginosa Biofilms

    • c-di-GMP

      • c-di-GMP Regulation of SCVs

      • Autoaggregation and c-di-GMP

      • Motility

      • Environmental Stimuli and Targets of c-di-GMP Signaling

    • QS and P. aeruginosa Biofilms

      • Effect of Nutrient on QS Regulation of Biofilm Formation

      • QS, Central Metabolism and Biofilm Gradients

      • QS and Chronic Infection

  • Biofilm/Eukaryote Interactions

    • Clinical P. aeruginosa Biofilm Infections

      • Chronic Lung Infections in CF

      • Conversion to Mucoidy and Anaerobic Growth

      • Phenotypic and Genotypic Analyses of P. aeruginosa in CF Infection

    • Similarities Across P. aeruginosa Biofilm–Host Interactions

      • Interactions with Host Immune Cells

      • Interactions with Free-living Protozoa

      • Interactions with Fungi

  • Future Perspectives

  • References