Chapter 2. Directed Evolution as a Means to Engineer Enantioselective Enzymes

  1. Prof. Vicente Gotor2,
  2. Prof. Ignacio Alfonso3 and
  3. Dr. Eduardo García-Urdiales4
  1. Manfred T. Reetz

Published Online: 17 JUN 2008

DOI: 10.1002/9783527622481.ch2

Asymmetric Organic Synthesis with Enzymes

Asymmetric Organic Synthesis with Enzymes

How to Cite

Reetz, M. T. (2008) Directed Evolution as a Means to Engineer Enantioselective Enzymes, in Asymmetric Organic Synthesis with Enzymes (eds V. Gotor, I. Alfonso and E. García-Urdiales), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527622481.ch2

Editor Information

  1. 2

    Universidad de Oviedo, Facultad de Química, Departamento de Química Orgánica e Inorgánica, Julian Clavería 6, 33006 Oviedo, Spain

  2. 3

    Dept.de Química Orgánica/Inorg, Universidad Jaume I, Campus del Riu Sec, 12071 Castellón, Spain

  3. 4

    EMBL - Biocomputing, Meyerhofstr. 1, 69117 Heidelberg, Germany

Author Information

  1. Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany

Publication History

  1. Published Online: 17 JUN 2008
  2. Published Print: 20 FEB 2008

ISBN Information

Print ISBN: 9783527318254

Online ISBN: 9783527622481

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Keywords:

  • asymmetric catalysis;
  • directed evolution;
  • enzyme engineering;
  • enantioselectivity;
  • high throughput assays;
  • molecular biological methods;
  • mutagenesis

Summary

This chapter contains sections titled:

  • Introduction

  • Molecular Biological Methods for Mutagenesis

  • High-throughput Screening Methods for Enantioselectivity

  • Examples of Enhancing the Enantioselectivity of Enzymes by Directed Evolution

  • Conclusions and Perspectives

  • References