Chapter 1. Single Cell Expression Profiling: Transcript and Protein Analyses in Isolated Higher Plant Gametes and Zygotes

  1. Prof. Dr. Günter Kahl2,3,
  2. Prof. Dr. Khalid Meksem4
  1. Stefan Scholten,
  2. Erhard Kranz

Published Online: 11 JUN 2008

DOI: 10.1002/9783527622542.ch1

The Handbook of Plant Functional Genomics: Concepts and Protocols

The Handbook of Plant Functional Genomics: Concepts and Protocols

How to Cite

Scholten, S. and Kranz, E. (2008) Single Cell Expression Profiling: Transcript and Protein Analyses in Isolated Higher Plant Gametes and Zygotes, in The Handbook of Plant Functional Genomics: Concepts and Protocols (eds G. Kahl and K. Meksem), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527622542.ch1

Editor Information

  1. 2

    Mohrmühlgasse 3, 63500 Seligenstadt, Germany

  2. 3

    GenXPro GmbH, Frankfurt Innovation Center Biotechnology (FIZ), Altenhöferallee 3, 60438 Frankfurt am Main, Germany

  3. 4

    Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901-4415, USA

Author Information

  1. Universität Hamburg, Biozentrum Klein Flottbek und Botanischer Garten, Entwicklungsbiologie und Biotechnologie, Ohnhorststr. 18, 22609 Hamburg, Germany

Publication History

  1. Published Online: 11 JUN 2008
  2. Published Print: 10 JUN 2008

ISBN Information

Print ISBN: 9783527318858

Online ISBN: 9783527622542

SEARCH

Keywords:

  • single cell expression profiling;
  • in vitro fertilization;
  • Reverse transcriptase polymerase chain reaction (RT-PCR);
  • multiplex reaction;
  • cDNA library;
  • microdissection

Summary

This chapter contains sections titled:

  • Introduction

  • Microdissection, Cell Isolation

  • In Vitro Fertilization

  • Techniques for Molecular Analyses of Single Cell Types

  • Analyses of Protein Expression

  • Prospects

  • References