Chapter 12. TILLING: A Reverse Genetics and a Functional Genomics Tool in Soybean

  1. Prof. Dr. Günter Kahl4,5,
  2. Prof. Dr. Khalid Meksem6
  1. Prof. Dr. Khalid Meksem6,
  2. Shiming Liu6,
  3. Xiao Hong Liu1,
  4. Aziz Jamai2,
  5. Melissa Goellner Mitchum1,
  6. Abdelhafid Bendahmane3,
  7. Tarik El-Mellouki6

Published Online: 11 JUN 2008

DOI: 10.1002/9783527622542.ch12

The Handbook of Plant Functional Genomics: Concepts and Protocols

The Handbook of Plant Functional Genomics: Concepts and Protocols

How to Cite

Meksem, K., Liu, S., Liu, X. H., Jamai, A., Mitchum, M. G., Bendahmane, A. and El-Mellouki, T. (2008) TILLING: A Reverse Genetics and a Functional Genomics Tool in Soybean, in The Handbook of Plant Functional Genomics: Concepts and Protocols (eds G. Kahl and K. Meksem), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527622542.ch12

Editor Information

  1. 4

    Mohrmühlgasse 3, 63500 Seligenstadt, Germany

  2. 5

    GenXPro GmbH, Frankfurt Innovation Center Biotechnology (FIZ), Altenhöferallee 3, 60438 Frankfurt am Main, Germany

  3. 6

    Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901-4415, USA

Author Information

  1. 1

    University of Missouri-Columbia, Division of Plant Sciences, 371H Life Sciences Center, Columbia, MO, 65211-7310, USA

  2. 2

    Dartmouth College, Department of Biological Sciences, Hanover, NH, 03755-3576, USA

  3. 3

    DR2 INRA, INRA/CNRS-URGV, 2 Rue Gaston Crémieux CP 5708, 91057 EVRY Cedex, France

  4. 6

    Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901-4415, USA

Publication History

  1. Published Online: 11 JUN 2008
  2. Published Print: 10 JUN 2008

ISBN Information

Print ISBN: 9783527318858

Online ISBN: 9783527622542

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Keywords:

  • Targeting Induced Local Lesion IN Genomes (TILLING);
  • mismatch detection enzymes;
  • functional genomic tool;
  • Ethlymethanesulfonate (EMS) (mutagenesis?);
  • reverse genetics;
  • Codons Optimized to Deliver Deleterious Lesions (CODDLe)

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and Protocols

  • Applications of TILLING to Soybean

  • Discussion and Perspectives

  • References