Chapter 5. Gene Identification Signature-Paired End diTagging (GIS-PET): A Technology for Transcriptome Characterization

  1. Prof. Dr. Günter Kahl2,3,
  2. Prof. Dr. Khalid Meksem4
  1. Patrick Ng,
  2. Yen-Ling Lee,
  3. Chia-Lin Wei,
  4. Yijun Ruan

Published Online: 11 JUN 2008

DOI: 10.1002/9783527622542.ch5

Book Title

The Handbook of Plant Functional Genomics: Concepts and Protocols

The Handbook of Plant Functional Genomics: Concepts and Protocols

Additional Information

How to Cite

Ng, P., Lee, Y.-L., Wei, C.-L. and Ruan, Y. (2008) Gene Identification Signature-Paired End diTagging (GIS-PET): A Technology for Transcriptome Characterization, in The Handbook of Plant Functional Genomics: Concepts and Protocols (eds G. Kahl and K. Meksem), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527622542.ch5

Editor Information

  1. 2

    Mohrmühlgasse 3, 63500 Seligenstadt, Germany

  2. 3

    GenXPro GmbH, Frankfurt Innovation Center Biotechnology (FIZ), Altenhöferallee 3, 60438 Frankfurt am Main, Germany

  3. 4

    Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901-4415, USA

Author Information

  1. GENOME, Genome Institute of Singapore, 60 Bipolis Street, Singapore 138672, Singapore

Publication History

  1. Published Online: 11 JUN 2008
  2. Published Print: 10 JUN 2008

ISBN Information

Print ISBN: 9783527318858

Online ISBN: 9783527622542

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CHAPTER TOOLS

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Keywords:

  • Gene identification–paired end ditagging (GIS-PET);
  • transcriptome characterization;
  • full length cDNA (flcDNA);
  • DNA-tagging;
  • splicing

Summary

This chapter contains sections titled:

  • Introduction

  • Protocol

  • Data Analysis

  • Discussion

  • Perspectives

  • References

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