Chapter 1. Fluorescent Sterols for the Study of Cholesterol Trafficking in Living Cells

  1. Prof. Dr. Lawrence W. Miller
  1. Avery L. McIntosh1,
  2. Huan Huang1,
  3. Barbara P. Atshaves1,
  4. Stephan M. Storey1,
  5. Adalberto M. Gallegos2,
  6. Thomas A. Spencer3,
  7. Robert Bittman4,
  8. Yoshiko Ohno-Iwashita5,
  9. Ann B. Kier2 and
  10. Friedhelm Schroeder1

Published Online: 29 SEP 2008

DOI: 10.1002/9783527623099.ch1

Probes and Tags to Study Biomolecular Function: for Proteins, RNA, and Membranes

Probes and Tags to Study Biomolecular Function: for Proteins, RNA, and Membranes

How to Cite

McIntosh, A. L., Huang, H., Atshaves, B. P., Storey, S. M., Gallegos, A. M., Spencer, T. A., Bittman, R., Ohno-Iwashita, Y., Kier, A. B. and Schroeder, F. (2008) Fluorescent Sterols for the Study of Cholesterol Trafficking in Living Cells, in Probes and Tags to Study Biomolecular Function: for Proteins, RNA, and Membranes (ed L. W. Miller), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527623099.ch1

Editor Information

  1. University of Illinois at Chicago, Department of Chemistry, 845 West Taylor Street, MC 111, Chicago, IL 60607-7061, USA

Author Information

  1. 1

    Texas A&M University, Department of Physiology and Pharmacology, TVMC, College Station, TX 77843-4466, USA

  2. 2

    Texas A&M University, Department of Pathobiology, TVMC, College Station, TX 77843-4466, USA

  3. 3

    Dartmouth College, Department of Chemistry, Hanover, NH 03755-1812, USA

  4. 4

    Queens College, Department of Chemistry and Biochemistry, CUNY, Flushing, NY 11367-1597, USA

  5. 5

    Tokyo Metropolitan Institute of Gerontology, Biomembrane Research Group, Tokyo 173-0015, Japan

Publication History

  1. Published Online: 29 SEP 2008
  2. Published Print: 23 JUL 2008

ISBN Information

Print ISBN: 9783527315666

Online ISBN: 9783527623099

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Keywords:

  • cholesterol;
  • dehydroergosterol;
  • fluorescent sterols;
  • multiphoton microscopy;
  • confocal microscopy

Summary

This chapter contains sections titled:

  • Introduction

  • Methods for Imaging Fluorescent Sterols in Living Cells: Confocal and Multiphoton Laser Scanning Microscopy

  • Cholesterol Structure and Distribution in Membranes

  • NBD-Cholesterol

  • Dansyl-Cholesterol

  • BODIPY-Cholesterol

  • Dehydroergosterol (DHE)

  • 22-(p-Benzoylphenoxy)-23,24-bisnorcholan-5-en-3β-ol (FCBP) Photoactivatable Sterol

  • BCθ

  • Conclusion

  • References