Chapter 8. Quantitative Distance and Position Measurement using Single-Molecule FRET

  1. Prof. Dr. Christoph Bräuchle1,
  2. Prof. Dr. Don C. Lamb1,2,3 and
  3. Prof. Dr. Jens Michaelis4
  1. Prof. Dr. Jens Michaelis

Published Online: 14 DEC 2009

DOI: 10.1002/9783527628360.ch8

Single Particle Tracking and Single Molecule Energy Transfer

Single Particle Tracking and Single Molecule Energy Transfer

How to Cite

Michaelis, J. (2009) Quantitative Distance and Position Measurement using Single-Molecule FRET, in Single Particle Tracking and Single Molecule Energy Transfer (eds C. Bräuchle, D. C. Lamb and J. Michaelis), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527628360.ch8

Editor Information

  1. 1

    Ludwig-Maximilians-Universität München, Department of Chemistry and Biochemistry, Center for Nanoscience (CeNS), Butenandtstrasse 11, 81377 München, Germany

  2. 2

    Munich Center for Integrated Protein Science (CiPSM), Butenandtstrasse 11, 81377 München, Germany

  3. 3

    University of Illinois, Department of Physics, Urbana, IL 61801, USA

  4. 4

    Ludwig-Maximilians-Universität München, Department of Chemistry and Biochemistry, Butenandtstrasse 11, 81377 München, Germany

Author Information

  1. Ludwig-Maximilians-Universität München, Department of Chemistry and Biochemistry, Butenandtstrasse 11, 81377 München, Germany

Publication History

  1. Published Online: 14 DEC 2009
  2. Published Print: 11 NOV 2009

ISBN Information

Print ISBN: 9783527322961

Online ISBN: 9783527628360

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Keywords:

  • Distance determination;
  • nanopositioning system;
  • orientational uncertainty;
  • RNA polymerase;
  • molecular ruler;
  • multiparameter FRET;
  • position determination;
  • Bayesian analysis

Summary

This chapter contains sections titled:

  • Introduction

  • Fundamentals of FRET

  • FRET as a Spectroscopic Ruler: Initial Experiments and Limitations

  • Measuring the Quantum Yield

  • The Orientation of Donor and Acceptor Molecules

  • Accurate FRET Measurements Using Fluorescence Correlation Spectroscopy

  • FRET-Based Triangulation and the Nanopositioning System

  • Conclusions and Outlook

  • References