Chapter 5.1. Stretching and Rupturing Single Covalent and Associating Macromolecules by AFM-Based Single-Molecule Force Spectroscopy

  1. Dr. Renate Förch3,
  2. Prof. Dr. Holger Schönherr4 and
  3. Dr. A. Tobias A. Jenkins5
  1. Marina I. Giannotti1,
  2. Weiqing Shi2,
  3. Shan Zou2,
  4. Prof. Dr. Holger Schönherr4 and
  5. G. Julius Vancso2

Published Online: 9 SEP 2009

DOI: 10.1002/9783527628599.ch20

Surface Design: Applications in Bioscience and Nanotechnology

Surface Design: Applications in Bioscience and Nanotechnology

How to Cite

Giannotti, M. I., Shi, W., Zou, S., Schönherr, H. and Vancso, G. J. (2009) Stretching and Rupturing Single Covalent and Associating Macromolecules by AFM-Based Single-Molecule Force Spectroscopy, in Surface Design: Applications in Bioscience and Nanotechnology (eds R. Förch, H. Schönherr and A. T. A. Jenkins), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527628599.ch20

Editor Information

  1. 3

    Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

  2. 4

    University of Siegen, Department of Physical Chemistry, Adolf-Reichwein-Straße 2, 57076 Siegen, Germany

  3. 5

    University of Bath, Department of Chemistry, Bath BA2 7AY, United Kingdom

Author Information

  1. 1

    University of Barcelona / CIBER–BBN, Institut de Bioenginyeria de Catalunya (IBEC), Edificio Hélix PCB, C/ Baldiri Reixac, 15–21, 08028 Barcelona, Spain

  2. 2

    University of Twente, MESA+ Institute for Nanotechnology, Department of Materials Science and Technology of Polymers, P.O. Box 217, 7500 AE Enschede, The Netherlands

  3. 4

    University of Siegen, Department of Physical Chemistry, Adolf-Reichwein-Straße 2, 57076 Siegen, Germany

Publication History

  1. Published Online: 9 SEP 2009
  2. Published Print: 12 JUN 2009

ISBN Information

Print ISBN: 9783527407897

Online ISBN: 9783527628599

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Keywords:

  • AFM-based single-molecule force spectroscopy;
  • stretching macromolecules;
  • rupturing macromolecules;
  • single-macromolecular motor;
  • host-guest complexes rupturing;
  • supramolecular polymers

Summary

Due to the high sensitivity of force detection and molecular displacement by atomic force microscopy (AFM), force-extension curves of single macromolecules can be monitored and rupture forces required to break single supramolecular bonds can be measured with high precision using AFM-based single-molecule force spectroscopy (AFM-SMFS). AFM-SMFS allows one to characterize the elasticity and conformational changes of individual macromolecules under stress, and has been extensively used for manipulation and mechanical characterization of biomacromolecules like DNA, proteins, polysaccharides, as well as synthetic polymers. In this chapter we first review the applications of AFM-SMFS for the construction and performance assessment of an artificial macromolecular motor based on a redox-responsive polymer, poly(dimethyl ferrocenylsilane). We then discuss the conformational transitions and H-bond-stabilized superstructures in hyaluronan polysaccharide individual chains. Finally, an overview of bond rupture of H-bonded supramolecular polymers at the single-molecule level is given. With these examples we aim at illustrating the potential of AFM-SMFS to explore molecular mechanics of macromolecular species of different nature at the single-chain level.