3. Interaction of Pulsed Light with Molecules: Photochemical and Photophysical Effects

  1. Dr. Francesco S. Pavone
  1. Gereon Hüttmann

Published Online: 4 OCT 2010

DOI: 10.1002/9783527632053.ch3

Laser Imaging and Manipulation in Cell Biology

Laser Imaging and Manipulation in Cell Biology

How to Cite

Hüttmann, G. (2010) Interaction of Pulsed Light with Molecules: Photochemical and Photophysical Effects, in Laser Imaging and Manipulation in Cell Biology (ed F. S. Pavone), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527632053.ch3

Editor Information

  1. University of Florence, LENS, European Laboratory for Non-Linear Spectroscopy, Via Nello Carrara 1, Polo Scientifico, I-50019 Sesto Fiorentino, Florence, Italy

Author Information

  1. University of Lübeck, Institute of Biomedical Optics, Peter-Monnik-Weg 4, 23562 Lübeck, Germany

Publication History

  1. Published Online: 4 OCT 2010
  2. Published Print: 13 OCT 2010

ISBN Information

Print ISBN: 9783527409297

Online ISBN: 9783527632053

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Keywords:

  • biomedical applications;
  • biomolecules;
  • interaction mechanisms;
  • multiphoton absorption;
  • photochemical effects;
  • photophysical effects;
  • photophysics;
  • pulsed light

Summary

Pulsed irradiation allows significantly higher irradiances or photon flux density, because the photons are concentrated over certain time period. Consequently, excited state absorption and bleaching of the ground state may change the photophysical behavior. The interaction cross-sections, and kinetics of the involved states have a significant influence on the photophysical and photochemical behavior. This chapter discusses relevant photophysical properties of molecules with special emphasis on the dynamics between states. It also discusses sequencing the effects of ground state bleaching and excited state absorption, which dominate the interaction in the kW and MW range. The chapter describes multiphoton excitation via virtual states that are dominant in the GW and TW range. It covers relevance of the interaction of pulsed light with molecules for biomedical imaging and optical manipulation of biomolecules and tissues. The chapter presents the basics of photophysics that are necessary to understand the interaction of pulsed light with molecules.

Controlled Vocabulary Terms

biomedical imaging; molecular biophysics; radiation effects