25. The Micromics Revolution: MicroRNA-Mediated Approaches to Develop Stress-Resistant Crops

  1. Dr. Narendra Tuteja2,3,
  2. Dr. Sarvajeet Singh Gill2,4,
  3. Prof. Antonio F. Tiburcio5 and
  4. Dr. Renu Tuteja2
  1. Camilo López and
  2. Aacute;lvaro L. Pérez-Quintero

Published Online: 30 MAR 2012

DOI: 10.1002/9783527632930.ch25

Improving Crop Resistance to Abiotic Stress, Volume 1 & Volume 2

Improving Crop Resistance to Abiotic Stress, Volume 1 & Volume 2

How to Cite

López, C. and Pérez-Quintero, A. L. (2012) The Micromics Revolution: MicroRNA-Mediated Approaches to Develop Stress-Resistant Crops, in Improving Crop Resistance to Abiotic Stress, Volume 1 & Volume 2 (eds N. Tuteja, S. S. Gill, A. F. Tiburcio and R. Tuteja), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527632930.ch25

Editor Information

  1. 2

    International Centre for Genetic Engineering and Biotechnology Plant Molecular Biology Group, Aruna Asaf Ali Marg, New Delhi 110 067, India

  2. 3

    MD University, Centre for Biotechnology, Rohtak 124 001, Haryana, India

  3. 4

    Aligarh Muslim University, Department of Botany, Aligarh 202 002, Uttar Pradesh, India

  4. 5

    Universitat de Barcelona, Unitat de Fisiologia Vegetal, Facultat de Farmàcia, Av. Joan XXIII, S/N, 08028 Barcelona, Spain

Author Information

  1. Universidad Nacional de Colombia, Biology Department, Street Cra 30 No 45-03, Bogotá DC, Colombia

Publication History

  1. Published Online: 30 MAR 2012
  2. Published Print: 14 MAR 2012

ISBN Information

Print ISBN: 9783527328406

Online ISBN: 9783527632930

SEARCH

Keywords:

  • abiotic stress;
  • bacteria;
  • microRNAs;
  • artificial microRNAs;
  • viruses

Summary

In the past two decades, small silencing RNAs (ssRNAs or sRNAs) have emerged as one of the most exciting and revolutionary discoveries in molecular biology. ssRNAs are noncoding molecules used by eukaryotes in transcriptional and posttranscriptional gene silencing, endogenous mechanisms for gene regulation and defense against invasive nucleic acids. MicroRNAs (miRNAs), a type of ssRNAs, play a pivotal role in endogenous regulation of gene expression via cleavage or translational repression of complementary “target” mRNA molecules. Plant miRNAs were first described in Arabidopsis in 2002 and now have been identified in more than 120 plant species (including crops and model species); thus, plant miRNA research is one of the fastest growing fields in molecular biology. The first families of miRNAs identified were known to mainly target transcription factors and were involved in growth and development regulation. The initial tools for miRNA identification were often time consuming, expensive, and difficult. In the last few years, various “omics” approaches have been developed to study miRNAs, allowing the spread of miRNA studies to a larger number of plants and the discovery of novel miRNAs and their functions. Notably, various miRNAs are now known to play a role in biotic and abiotic stress, which has led researchers to consider them as a promising tool to develop stress-resistant crops. In this chapter, we review the role miRNAs play in the way plants react to biotic and abiotic stress. We highlight the new methodologies being used to discover miRNAs and to understand their functions in plant responses to environmental stimuli. We also discuss and emphasize the possibilities of generating crop plants with increased stress tolerance using microRNA-mediated technologies.