9. Comparison of Immunophenotyping and Rare Cell Detection by Slide-Based Imaging Cytometry and Flow Cytometry

  1. Prof. Valery V. Tuchin4,5
  1. József Bocsi1,2,
  2. Anja Mittag1,3 and
  3. Attila Tárnok1,2

Published Online: 5 APR 2011

DOI: 10.1002/9783527634286.ch9

Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

How to Cite

Bocsi, J., Mittag, A. and Tárnok, A. (2011) Comparison of Immunophenotyping and Rare Cell Detection by Slide-Based Imaging Cytometry and Flow Cytometry, in Advanced Optical Flow Cytometry: Methods and Disease Diagnoses (ed V. V. Tuchin), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527634286.ch9

Editor Information

  1. 4

    Saratov State University, Optics and Biophotonics, Department and Research-Educational Institute of Optics and Biophotonics, 83 Astrakhanskaya St., Saratov 410012, Russia

  2. 5

    Laboratory on Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control of RAS, 24, Rabochaya st., Saratov 410028, Russia

Author Information

  1. 1

    University of Leipzig, Department of Paediatric Cardiology, Heart Centre, Strümpellstr. 39, 04275 Leipzig, Germany

  2. 2

    University of Leipzig, LIFE - Leipzig Research Center for Civilization Diseases, Faculty of Medicine, Philipp-Rosenthal-Str. 27, 04103 Leipzig, Germany

  3. 3

    University of Leipzig, Translational Centre for Regenerative Medicine (TRM), Leipzig, Philipp-Rosenthal-Str. 55, 04103 Leipzig, Germany

Publication History

  1. Published Online: 5 APR 2011
  2. Published Print: 20 APR 2011

ISBN Information

Print ISBN: 9783527409341

Online ISBN: 9783527634286

SEARCH

Keywords:

  • slide-based cytometry (SBC);
  • laser scanning cytometry (LSC);
  • flow cytometry (FCM);
  • scanning fluorescence microscopy (SFM);
  • bleaching;
  • quantum dots (QD);
  • immunophenotyping;
  • fluorescence analysis;
  • tumor cells;
  • apoptosis

Summary

This chapter contains sections titled:

  • Introduction

  • Comparison of Four-Color CD4/CD8 Leukocyte Analysis by SFM and FCM Using Qdot Staining

  • Comparison of Leukocyte Subtyping by Multiparametric Analysis with LSC and FCM

  • Absolute and Relative Tumor Cell Frequency Determinations

  • Analysis of Drug-Induced Apoptosis in Leukocytes by Propidium Iodide

  • Conclusion

  • Acknowledgment

  • References