12. Detection of Differentially Modified Pathogen Proteins by Western Blot after 2D Gel Electrophoresis and Identification by MALDI-TOF/TOF

  1. Prof. Jiri Stulik2,3,4,
  2. Prof. Rudolf Toman5,
  3. Prof. Patrick Butaye6,7 and
  4. Dr. Robert G. Ulrich8
  1. Fred Fack,
  2. Julia Kessler,
  3. Patrick Pirrotte,
  4. Jacques Kremer,
  5. Dominique Revets,
  6. Wim Ammerlaan and
  7. Claude P. Muller

Published Online: 30 SEP 2011

DOI: 10.1002/9783527638192.ch12

BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity

BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity

How to Cite

Fack, F., Kessler, J., Pirrotte, P., Kremer, J., Revets, D., Ammerlaan, W. and Muller, C. P. (2011) Detection of Differentially Modified Pathogen Proteins by Western Blot after 2D Gel Electrophoresis and Identification by MALDI-TOF/TOF, in BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity (eds J. Stulik, R. Toman, P. Butaye and R. G. Ulrich), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527638192.ch12

Editor Information

  1. 2

    Ministry of Defence, Faculty of Military Health Service, Trebesska 1575, 500 01 Hradec Králové, Czech Republic

  2. 3

    Institute of Molecular Pathology, FMHS UO, Trebesska 1575, 500 01 Hradec Králové, Czech Republic

  3. 4

    Centre of Advanced Studies, Faculty of Military Health Sciences, Trebesska 1575, 500 01 Hradec Králové, Czech Republic

  4. 5

    Slovak Academy of Sciences, Institute of Virology, Laboratory for Diagnosis and Prevention of Rickettsial and Chlamydial Infections, Dubravska cesta 9, 845 05 Bratislava 45, Slovak Republic

  5. 6

    Veterimary and Agrochemical Research Centre, Department of Bacteriology and Immunology, VAR-CODA-CERVA, Groeselenberg 99, 1180 Bruxelles, Belgium

  6. 7

    Ghent University, Faculty of Veterinary Medicine, Department of Pathology, Bacteriology, and Poultry Diseases, Salisburylaan 133, 9820 Merelbeke, Belgium

  7. 8

    United States Army Medical Research, Institute of Infectious Diseases, Department of Immunology, 1425 Porter Street, Frederick, MD 21702, USA

Author Information

  1. Institute of Immunology, Centre de Recherche Public de la, Santé/National Public Health Institute, 20A, rue Auguste Lumière, 1950 Luxembourg, Luxembourg

Publication History

  1. Published Online: 30 SEP 2011
  2. Published Print: 21 SEP 2011

ISBN Information

Print ISBN: 9783527327805

Online ISBN: 9783527638192

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Keywords:

  • pathogen protein;
  • 2D Western blot;
  • fluorescence;
  • multiplex antibody detection

Summary

This chapter contains sections titled:

  • Introduction

  • Materials and Methods

  • Results

  • Discussion

  • References