15. Subtype and Toxin Variant Identification of Botulinum Neurotoxin Type a Using Proteomics Techniques

  1. Prof. Jiri Stulik3,4,5,
  2. Prof. Rudolf Toman6,
  3. Prof. Patrick Butaye7,8 and
  4. Dr. Robert G. Ulrich9
  1. Suzanne R. Kalb1,
  2. Jakub Baudys1,
  3. Theresa J. Smith2,
  4. James L. Pirkle1 and
  5. John R. Barr1

Published Online: 30 SEP 2011

DOI: 10.1002/9783527638192.ch15

BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity

BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity

How to Cite

Kalb, S. R., Baudys, J., Smith, T. J., Pirkle, J. L. and Barr, J. R. (2011) Subtype and Toxin Variant Identification of Botulinum Neurotoxin Type a Using Proteomics Techniques, in BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity (eds J. Stulik, R. Toman, P. Butaye and R. G. Ulrich), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527638192.ch15

Editor Information

  1. 3

    Ministry of Defence, Faculty of Military Health Service, Trebesska 1575, 500 01 Hradec Králové, Czech Republic

  2. 4

    Institute of Molecular Pathology, FMHS UO, Trebesska 1575, 500 01 Hradec Králové, Czech Republic

  3. 5

    Centre of Advanced Studies, Faculty of Military Health Sciences, Trebesska 1575, 500 01 Hradec Králové, Czech Republic

  4. 6

    Slovak Academy of Sciences, Institute of Virology, Laboratory for Diagnosis and Prevention of Rickettsial and Chlamydial Infections, Dubravska cesta 9, 845 05 Bratislava 45, Slovak Republic

  5. 7

    Veterimary and Agrochemical Research Centre, Department of Bacteriology and Immunology, VAR-CODA-CERVA, Groeselenberg 99, 1180 Bruxelles, Belgium

  6. 8

    Ghent University, Faculty of Veterinary Medicine, Department of Pathology, Bacteriology, and Poultry Diseases, Salisburylaan 133, 9820 Merelbeke, Belgium

  7. 9

    United States Army Medical Research, Institute of Infectious Diseases, Department of Immunology, 1425 Porter Street, Frederick, MD 21702, USA

Author Information

  1. 1

    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, N.E., Atlanta, GA 30341, USA

  2. 2

    United States Army Medical Research, Institute of Infectious Diseases, Integrated Toxicology, Ft. Detrick, MD 21702, USA

Publication History

  1. Published Online: 30 SEP 2011
  2. Published Print: 21 SEP 2011

ISBN Information

Print ISBN: 9783527327805

Online ISBN: 9783527638192

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Keywords:

  • botulinum;
  • neurotoxin;
  • variant;
  • subtype;
  • identification;
  • proteomics

Summary

This chapter contains sections titled:

  • Introduction

  • Botulinum Neurotoxins

  • Differentiation of Botulinum Neurotoxins

  • Amino Acid Sequence Identification Using Proteomics

  • Extraction of BoNT from Complex Matrices

  • Identification of BoNT Serotype with Proteomics

  • Identification of BoNT/A Subtype with Proteomics

  • Identification of BoNT/A1 Strain with Proteomics

  • Conclusions

  • Disclaimer

  • References