26. 13C Direct Detection NMR
- Prof. Dr. Ivano Bertini,
- Kathleen S. McGreevy,
- Prof. Giacomo Parigi
Published Online: 21 MAR 2012
DOI: 10.1002/9783527644506.ch26
Copyright © 2012 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
NMR of Biomolecules: Towards Mechanistic Systems Biology
Additional Information
How to Cite
Felli, I. C. and Pierattelli, R. (2012) 13C Direct Detection NMR, in NMR of Biomolecules: Towards Mechanistic Systems Biology (eds I. Bertini, K. S. McGreevy and G. Parigi), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527644506.ch26
Editor Information
University of Florence, Department of Chemistry and Magnetic Resonance Center (CERM), Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy
Publication History
- Published Online: 21 MAR 2012
- Published Print: 14 MAR 2012
ISBN Information
Print ISBN: 9783527328505
Online ISBN: 9783527644506
- Summary
- Chapter
- References
Keywords:
- 13C direct detection;
- NMR;
- biomolecule;
- proton-detected NMR;
- heteronuclei;
- general procedure
Summary
Thanks to improvements in instrumental sensitivity as well as the development of a suite of new experiments, 13C direct detection NMR offers a useful tool for biomolecular applications. It can generally be applied in parallel to proton-detected NMR, providing additional and complementary data. In a variety of cases, as a result of the intrinsically different properties of the heteronuclei with respect to proton nuclei, it also enables the recovery of information that is lost or difficult to access through proton detection. This chapter addresses the several areas of biomolecular NMR where experiments based on 13C direct detection are particularly useful. The entire experimental NMR strategy, the key instrumental aspects, and the general procedures for the setup of NMR experiments are outlined.