16. DGS (Ditag Genome Scanning) – A Restriction-Based Paired-End Sequencing Approach for Genome Structural Analysis

  1. Dr. Matthias Harbers3,4 and
  2. Prof. Dr. Günter Kahl5,6,7
  1. Jun Chen1,
  2. Yeong C. Kim2 and
  3. San Ming Wang2

Published Online: 23 JAN 2012

DOI: 10.1002/9783527644582.ch16

Tag-Based Next Generation Sequencing

Tag-Based Next Generation Sequencing

How to Cite

Chen, J., Kim, Y. C. and Wang, S. M. (2011) DGS (Ditag Genome Scanning) – A Restriction-Based Paired-End Sequencing Approach for Genome Structural Analysis, in Tag-Based Next Generation Sequencing (eds M. Harbers and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527644582.ch16

Editor Information

  1. 3

    4-2-6 Nishihara, Kashiwa-Shi, Chiba 277-0885, Japan

  2. 4

    DNAFORM Inc., Leading Venture Plaza 2, 75-1 Ono-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0046, Japan

  3. 5

    Mohrmühlgasse 3, 63500 Seligenstadt, Germany

  4. 6

    University of Frankfurt am Main Biocenter, Max-von-Lauestraße 9, 60439 Frankfurt am Main, Germany

  5. 7

    Frankfurt Biotechnology Innovation Center (FIZ), GenXPro Ltd, Altenhöferallee 3, 60438 Frankfurt am Main, Germany

Author Information

  1. 1

    Xiamen University, School of Life Sciences, Department of Ocean Biology, Xiamen, Fujian 361012, China

  2. 2

    University of Nebraska Medical Center, Department of Genetics, Cell Biology & Anatomy, 42nd and Emile, Omaha, NE 68198, USA

Publication History

  1. Published Online: 23 JAN 2012
  2. Published Print: 14 DEC 2011

ISBN Information

Print ISBN: 9783527328192

Online ISBN: 9783527644582

SEARCH

Keywords:

  • DGS (ditag genome scanning);
  • restriction-based paired-end sequencing;
  • genome structural analysis;
  • methods

Summary

The paired-end sequencing approach provides a powerful means for studying genome structural changes. We developed the DGS (ditag genome scanning) technique that collects the paired-end tags from restriction fragments. We established the detailed protocols for collecting the paired-end tag sequences using different next-generation sequencing platforms and developed an informatics pipeline for DGS data mapping analysis. Our applications of DGS in multiple genome analyses show that DGS provides a tool for genome structural studies with high specificity and versatile resolution.