18. HELP-Tagging: Tag-Based Genome-Wide Cytosine Methylation Profiling

  1. Dr. Matthias Harbers2,3 and
  2. Prof. Dr. Günter Kahl4,5,6
  1. Masako Suzuki and
  2. John M. Greally

Published Online: 23 JAN 2012

DOI: 10.1002/9783527644582.ch18

Tag-Based Next Generation Sequencing

Tag-Based Next Generation Sequencing

How to Cite

Suzuki, M. and Greally, J. M. (2011) HELP-Tagging: Tag-Based Genome-Wide Cytosine Methylation Profiling, in Tag-Based Next Generation Sequencing (eds M. Harbers and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527644582.ch18

Editor Information

  1. 2

    4-2-6 Nishihara, Kashiwa-Shi, Chiba 277-0885, Japan

  2. 3

    DNAFORM Inc., Leading Venture Plaza 2, 75-1 Ono-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0046, Japan

  3. 4

    Mohrmühlgasse 3, 63500 Seligenstadt, Germany

  4. 5

    University of Frankfurt am Main Biocenter, Max-von-Lauestraße 9, 60439 Frankfurt am Main, Germany

  5. 6

    Frankfurt Biotechnology Innovation Center (FIZ), GenXPro Ltd, Altenhöferallee 3, 60438 Frankfurt am Main, Germany

Author Information

  1. Albert Einstein College of Medicine, Center for Epigenomics, Department of Genetics, 1301 Morris Park Avenue, Bronx, NY 10461, USA

Publication History

  1. Published Online: 23 JAN 2012
  2. Published Print: 14 DEC 2011

ISBN Information

Print ISBN: 9783527328192

Online ISBN: 9783527644582

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Keywords:

  • HELP-tagging;
  • tag-based;
  • genome-wide;
  • cytosine methylation profiling;
  • methods;
  • applications

Summary

We describe a restriction enzyme-based approach to study cytosine methylation using massively parallel sequencing that balances cost efficiency with comprehensiveness in a way that is suitable for human disease studies of large patient cohorts.