20. Moving Towards Third-Generation Sequencing Technologies

  1. Dr. Matthias Harbers3,4 and
  2. Prof. Dr. Günter Kahl5,6,7
  1. Karolina Janitz1 and
  2. Michal Janitz2

Published Online: 23 JAN 2012

DOI: 10.1002/9783527644582.ch20

Tag-Based Next Generation Sequencing

Tag-Based Next Generation Sequencing

How to Cite

Janitz, K. and Janitz, M. (2011) Moving Towards Third-Generation Sequencing Technologies, in Tag-Based Next Generation Sequencing (eds M. Harbers and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527644582.ch20

Editor Information

  1. 3

    4-2-6 Nishihara, Kashiwa-Shi, Chiba 277-0885, Japan

  2. 4

    DNAFORM Inc., Leading Venture Plaza 2, 75-1 Ono-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0046, Japan

  3. 5

    Mohrmühlgasse 3, 63500 Seligenstadt, Germany

  4. 6

    University of Frankfurt am Main Biocenter, Max-von-Lauestraße 9, 60439 Frankfurt am Main, Germany

  5. 7

    Frankfurt Biotechnology Innovation Center (FIZ), GenXPro Ltd, Altenhöferallee 3, 60438 Frankfurt am Main, Germany

Author Information

  1. 1

    Hawkesbury Institute for the Environment, University of Western Sydney, Hawkesbury Campus, Locked Bag 1797, Penrith, NSW 2751, Australia

  2. 2

    University of New South Wales, School of Biotechnology and Biomolecular Sciences, Biological Sciences Building, Kensington, NSW 2052, Australia

Publication History

  1. Published Online: 23 JAN 2012
  2. Published Print: 14 DEC 2011

ISBN Information

Print ISBN: 9783527328192

Online ISBN: 9783527644582



  • third-generation sequencing technologies;
  • differences NGS – Sanger sequencing;
  • templates preparation;
  • perspectives


Within just the past few years, next-generation sequencing (NGS) platforms have reduced the cost of DNA sequencing by several orders of magnitude. With these lower costs, DNA sequencing is increasingly powerful as a single-data format onto which a broad range of biological processes can be projected for high-throughput molecular profiling. Although significant challenges remain, translating these powerful technologies into useful laboratory tests has great potential. This chapter provides a comparative analysis of currently available NGS techniques, including those of Illumina, 454/Roche, Applied Biosystems, and Helicos BioSciences, and addresses emerging techniques, such as Pacific Biosciences single-molecule sequencing and nanopore sequencing. Furthermore, very recent electronic sequencing from Ion Torrent is elaborated. The chapter discusses advantages and disadvantages of individual platforms, and those common to all techniques, including template preparation and genome enrichment.