12. Yeast Genome and Postgenomic Projects

  1. Prof. Dr. Horst Feldmann1,2

Published Online: 26 SEP 2012

DOI: 10.1002/9783527659180.ch12

Yeast: Molecular and Cell Biology, Second Edition

Yeast: Molecular and Cell Biology, Second Edition

How to Cite

Feldmann, H. (ed) (2012) Yeast Genome and Postgenomic Projects, in Yeast: Molecular and Cell Biology, Second Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527659180.ch12

Editor Information

  1. 1

    Adolf Butenandt Institute, Molecular Biology, Ludwig-Maximilians-Universität M¨nchen, Schillerstr. 44, 80336 M¨nchen, Germany

  2. 2

    Ludwig-Thoma-Strasse 22B, 85232 Bergkirchen, Germany

Publication History

  1. Published Online: 26 SEP 2012
  2. Published Print: 22 AUG 2012

ISBN Information

Print ISBN: 9783527332526

Online ISBN: 9783527659180

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Keywords:

  • yeastgenome;
  • postgenomicprojects;
  • yeast genome sequencing project;
  • yeast functional genomics;
  • yeast synthetic biology

Summary

• An enormous leap forward for yeast molecular biology was the determination of the complete yeast genome sequence finalized in 1996 and subsequent efforts to functionally annotate as many of the yeast proteins as feasible. By this endeavor, not only the yeast community gained fresh impetus, but also the information from the yeast genome could be used as a reference against which other genomes could be compared and analyzed. In the “postgenomic era,” new useful tools for genome profiling were developed and “conventional” approaches improved. These techniques include the “old” two-hybrid analysis to detect protein–protein interactions on a genome-wide scale as well as many procedures that have a priori have been newly developed to enable studies of genes, their products, their modifications, and their dynamics both with high throughput and on a nano-scale. One important technology set up right after the establishment of the yeast genome sequence was the microarray technique, which was neatly suited to investigate the complete yeast genome profile under varying conditions. New methods were invented as tools to identify interacting genes and proteins – ChIP and TAP. These methods are still in use in investigations of systems biology, aimed at the understanding of the multiscale organization of living systems and how quantitative behaviors of biological systems can be predicted given knowledge of their present state.

• To date, we have reached a solid knowledge in yeast genomics, proteomics, and metabolomics. Many examples are provided to illustrate the potentials of this simple eukaryote. Nonetheless, we have to admit that a considerable portion of the yeast genes have still to be typified as “unknown in function.”

• Therefore, the information obtained by sequencing a variety of genomes from yeast species evolutionarily related to S. cerevisiae (the Hemiascomycetous yeasts) opened a new field of “evolutionary genomics.” Comparison of the gene content in these organisms offered explanations why particular classes of genes have been selected during evolution for adaptation to individual habitats. On a genomic scale, gene duplications and rearrangements could be followed over a long evolutionary range (several hundred millions of years). These aspects are dealt with in Chapters 15 and 16.