8. Yeast Transport
- Prof. Dr. Horst Feldmann1,2
Published Online: 26 SEP 2012
Copyright © 2012 Wiley-VCH Verlag GmbH & Co. KGaA
Yeast: Molecular and Cell Biology, Second Edition
How to Cite
Feldmann, H. (ed) (2012) Yeast Transport, in Yeast: Molecular and Cell Biology, Second Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527659180.ch8
Adolf Butenandt Institute, Molecular Biology, Ludwig-Maximilians-Universität M¨nchen, Schillerstr. 44, 80336 M¨nchen, Germany
Ludwig-Thoma-Strasse 22B, 85232 Bergkirchen, Germany
- Published Online: 26 SEP 2012
- Published Print: 22 AUG 2012
Print ISBN: 9783527332526
Online ISBN: 9783527659180
- intracellular protein sorting;
- nuclear traffic;
- membrane transporters
• Even in a small cell, functional transport is of utmost importance – components manufactured at one site of the cell have to be quickly and reliably delivered to sites of use. This intracellular traffic has to obey logistics with absolute directionality, which is guaranteed by two or three types of targeting specificity. Most of the intracellular traffic occurs between membrane-surrounded organelles and the plasma membrane.
• The majority of the yeast proteins are synthesized at cytosolic ribosomes and then transferred into the ER to be folded or checked for correct folding. A comprehensive description is devoted to the processes and components underlying protein sorting and transport within the yeast cell, which includes aspects of protein traffic from the ER through the Golgi apparatus as the major modifier and distributor of proteins, towards the vacuolar network, as well as endocytosis, exocytosis, and retrieval pathways. In particular, the yeast system has contributed a wealth of data on tethering complexes, membrane mechanics in producing and absorbing transport vesicles, and the autophagic process.
• During the past few years, our understanding of the NPC and nuclear traffic has grown considerably. Convincing models for the mechanics of nuclear transport and for biosynthetic pathways of restoration or renewal of nuclear pores have been established.
• Ample information has also accumulated in yeast on membrane transporters, their composition, and their mode of action. Yeast is one of the few organisms for which complete descriptions and catalogs of the various types of transporters are available. We give preference to explain the channels and ATP-dependent transporters that carry important ions (protons, sodium, potassium, and calcium) in and out of the cell as well as between organelles. Likewise, the systems for cellular supply with heavy metal ions, required as cofactors for many enzymes, are described in some detail. An overview on important carriers of yeast nutrients (carbon and nitrogen compounds) is presented.
• Owing to the outstanding role of mitochondria in yeast metabolic life, a separate section is devoted to the transport of small molecules across the mitochondrial membrane. Peculiarities of the yeast mitochondria will emanate from a description of the electron transport chain, the respiratory chain, and the yeast ATP synthase.