5. Protein Engineering: Development of Novel Enzymes for the Improved Reduction of C[DOUBLE BOND]C Double Bonds

  1. Elisabetta Brenna
  1. Sabrina Kille1 and
  2. Manfred T. Reetz2

Published Online: 30 AUG 2013

DOI: 10.1002/9783527665785.ch05

Synthetic Methods for Biologically Active Molecules: Exploring the Potential of Bioreductions

Synthetic Methods for Biologically Active Molecules: Exploring the Potential of Bioreductions

How to Cite

Kille, S. and Reetz, M. T. (2013) Protein Engineering: Development of Novel Enzymes for the Improved Reduction of C[DOUBLE BOND]C Double Bonds, in Synthetic Methods for Biologically Active Molecules: Exploring the Potential of Bioreductions (ed E. Brenna), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527665785.ch05

Editor Information

  1. Politecnico di Milano, Via Mancinelli 7, 20131 Milano, Italy

Author Information

  1. 1

    Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470, Mülheim an der Ruhr, Germany

  2. 2

    Department of Chemistry, Philipps-University Marburg, Hans-Meerwein-Strasse, 35032, Marburg, Germany

Publication History

  1. Published Online: 30 AUG 2013
  2. Published Print: 23 OCT 2013

ISBN Information

Print ISBN: 9783527333875

Online ISBN: 9783527665785

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Keywords:

  • directed evolution;
  • iterative saturation mutagenesis;
  • old yellow enzymes;
  • protein-engineering studies;
  • rational design;
  • unsaturated ketones

Summary

This chapter contains sections titled:

  • Introduction

  • The Protein Engineering Process and Employed Mutagenesis Methods

  • Examples of Rational Design of Old Yellow Enzymes

  • Evolving Old Yellow Enzymes (OYEs)

  • Conclusions and Perspectives