6. Protein Engineering: Development of Novel Enzymes for the Improved Reduction of C[DOUBLE BOND]O Double Bonds

  1. Elisabetta Brenna
  1. Nobuya Itoh and
  2. Yoshihide Makino

Published Online: 30 AUG 2013

DOI: 10.1002/9783527665785.ch06

Synthetic Methods for Biologically Active Molecules: Exploring the Potential of Bioreductions

Synthetic Methods for Biologically Active Molecules: Exploring the Potential of Bioreductions

How to Cite

Itoh, N. and Makino, Y. (2013) Protein Engineering: Development of Novel Enzymes for the Improved Reduction of C[DOUBLE BOND]O Double Bonds, in Synthetic Methods for Biologically Active Molecules: Exploring the Potential of Bioreductions (ed E. Brenna), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527665785.ch06

Editor Information

  1. Politecnico di Milano, Via Mancinelli 7, 20131 Milano, Italy

Author Information

  1. Toyama Prefectural University, Biotechnology Research Center and Department of Biotechnology, Kurokawa 5180, Imizu, Toyama, 939-0398, Japan

Publication History

  1. Published Online: 30 AUG 2013
  2. Published Print: 23 OCT 2013

ISBN Information

Print ISBN: 9783527333875

Online ISBN: 9783527665785

SEARCH

Keywords:

  • chiral alcohol;
  • keto ester reductase;
  • LSADH;
  • organic synthesis;
  • phenylacetaldehyde reductase;
  • protein engineering

Summary

This chapter contains sections titled:

  • Introduction

  • Detailed Characterization of PAR

  • Detailed Characterization of LSADH

  • Engineering of PAR for Increasing Activity in 2-Propanol/Water Medium

  • Application of Whole-Cell Biocatalysts Possessing Mutant PARs and LSADH

  • Engineering of β-Keto Ester Reductase (KER) for Raising Thermal Stability and Stereoselectivity

  • New Approach for Engineering or Obtaining Useful ADHs/Reductases