3. Fluorescence Microscopy

  1. Prof. Dr. Ulrich Kubitscheck
  1. Jurek W. Dobrucki

Published Online: 4 APR 2013

DOI: 10.1002/9783527671595.ch3

Fluorescence Microscopy: From Principles to Biological Applications

Fluorescence Microscopy: From Principles to Biological Applications

How to Cite

Dobrucki, J. W. (2013) Fluorescence Microscopy, in Fluorescence Microscopy: From Principles to Biological Applications (ed U. Kubitscheck), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527671595.ch3

Editor Information

  1. Rheinische Friedrich Wilhelms-Universität Bonn, Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, Wegeler Strasse 12, 53115 Bonn, Germany

Author Information

  1. Jagiellonian University, Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, ul. Gronostajowa 7, 30-387 Kraków, Poland

Publication History

  1. Published Online: 4 APR 2013
  2. Published Print: 24 APR 2013

ISBN Information

Print ISBN: 9783527329229

Online ISBN: 9783527671595

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Keywords:

  • fluorescence;
  • microscopy;
  • fluorescence contrast;
  • fluorescence spectrum;
  • imaging;
  • CCD camera;
  • EMCCD;
  • ICCD;
  • CMOS;
  • binning;
  • PMT;
  • APD;
  • well depth;
  • optical filter;
  • AOTF;
  • confocal microscopy;
  • laser scanning cytometry;
  • photobleaching;
  • phototoxicity;
  • light diffraction

Summary

Fluorescence microscopy is a versatile technique that can be used to specifically label a selected structure in a cell or in a tissue fragment. If properly performed, it yields high-contrast images. Multicolor fluorescence signals can be linked to various cellular components and functional parameters in order to study fixed and live cells. High specificity and high sensitivity of detection can be achieved with modern labeling techniques, and sophisticated illumination and light detection equipment.