1. Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond

  1. Sergio Riva3 and
  2. Wolf-Dieter Fessner4
  1. Eva Garcia-Ruiz1,
  2. Diana M. Mate1,
  3. David Gonzalez-Perez1,
  4. Patricia Molina-Espeja1,
  5. Susana Camarero2,
  6. Angel T. Martínez2,
  7. Antonio O. Ballesteros1 and
  8. Miguel Alcalde1

Published Online: 27 JUN 2014

DOI: 10.1002/9783527682492.ch1

Cascade Biocatalysis : Integrating Stereoselective and Environmentally Friendly Reactions

Cascade Biocatalysis : Integrating Stereoselective and Environmentally Friendly Reactions

How to Cite

Garcia-Ruiz, E., Mate, D. M., Gonzalez-Perez, D., Molina-Espeja, P., Camarero, S., Martínez, A. T., Ballesteros, A. O. and Alcalde, M. (2014) Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond, in Cascade Biocatalysis : Integrating Stereoselective and Environmentally Friendly Reactions (eds S. Riva and W.-D. Fessner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527682492.ch1

Editor Information

  1. 3

    Istituto di Chimica del, Riconoscimento Molecolare, C. N. R., Via Mario Bianco 9, 20131 Milano, Italy

  2. 4

    Technische Universität, Darmstadt, Department of Organic, Chemistry and Biochemistry, Petersenstr. 22, D-64287 Darmstadt, Germany

Author Information

  1. 1

    Institute of Catalysis, CSIC, Department of Biocatalysis, C/Marie Curie n°2, Cantoblanco, 28049, Madrid, Spain

  2. 2

    CSIC, Centro de Investigaciones Biológicas, Ramiro de Maeztu 9, E-28040, Madrid, Spain

Publication History

  1. Published Online: 27 JUN 2014
  2. Published Print: 23 JUL 2014

ISBN Information

Print ISBN: 9783527335220

Online ISBN: 9783527682492

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Keywords:

  • oxidoreductase;
  • lignin;
  • directed evolution;
  • peroxidase;
  • laccase;
  • peroxygenase;
  • Saccharomyces cerevisiae;
  • white-rot fungi

Summary

The ligninolytic enzymatic consortium, formed mainly by nonspecific oxidoreductases (laccases, peroxidases, and H2O2-supplying oxidases), is a potentially powerful multipurpose tool for industrial and environmental biotechnology. In nature, these enzymes are typically produced by basidiomycete white-rot fungi that are involved in lignin decay. These enzymes have many potential applications in the field of green chemistry, including the production of biofuels, bioremediation, organic syntheses, pulp biobleaching, food and textile industries, and the design of bionanodevices, because of their broad substrate specificity, high redox potential, and minimal requirements. However, their practical use has been hampered by the lack of appropriate molecular instruments, including heterologous hosts for directed evolution, with which to improve their properties. Over the past 10 years, a wealth of directed evolution strategies in combination with hybrid approaches has emerged in order to adapt these oxidoreductases to the drastic conditions associated with many biotechnological settings (e.g., high temperatures, the presence of organic cosolvents, extreme pHs, the presence of inhibitors). This chapter summarizes all efforts and endeavors to convert these ligninolytic enzymes into useful biocatalysts by means of directed evolution from functional expression to stabilization and beyond.