2. New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors

  1. Sergio Riva2 and
  2. Wolf-Dieter Fessner3
  1. Erica Elisa Ferrandi,
  2. Daniela Monti and
  3. Sergio Riva

Published Online: 27 JUN 2014

DOI: 10.1002/9783527682492.ch2

Cascade Biocatalysis : Integrating Stereoselective and Environmentally Friendly Reactions

Cascade Biocatalysis : Integrating Stereoselective and Environmentally Friendly Reactions

How to Cite

Ferrandi, E. E., Monti, D. and Riva, S. (2014) New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors, in Cascade Biocatalysis : Integrating Stereoselective and Environmentally Friendly Reactions (eds S. Riva and W.-D. Fessner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527682492.ch2

Editor Information

  1. 2

    Istituto di Chimica del, Riconoscimento Molecolare, C. N. R., Via Mario Bianco 9, 20131 Milano, Italy

  2. 3

    Technische Universität, Darmstadt, Department of Organic, Chemistry and Biochemistry, Petersenstr. 22, D-64287 Darmstadt, Germany

Author Information

  1. Istituto di Chimica del Riconoscimento Molecolare, C. N. R., Via Mario Bianco 9, 20131, Milano, Italy

Publication History

  1. Published Online: 27 JUN 2014
  2. Published Print: 23 JUL 2014

ISBN Information

Print ISBN: 9783527335220

Online ISBN: 9783527682492

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Keywords:

  • nicotinammide cofactors;
  • dehydrogenases;
  • cofactor regeneration;
  • oxidation;
  • reduction

Summary

Owing to the high cost of the nicotinammide cofactors, cofactor regeneration is a critical issue in the synthetic exploitation of NAD(P)H-dependent dehydrogenases. Therefore, an efficient and economical cofactor regeneration system, allowing the use of catalytic amounts of the cofactor in the reaction, is mandatory for the industrial application of these biocatalysts.

In the past 50 years, several nicotinammide cofactor regeneration systems have been investigated, such as chemical, photochemical, electrochemical, biological, and mixed methods. Of them, the use of enzymatic recycling systems seems to be the most convenient strategy, even if presenting some limitations from a practical and economical point of view. In recent years, many progresses have been made to overcome these limitations, trying to improve the already established enzymatic systems or developing conceptually new approaches.

In this chapter, the recent advancements in enzymatic methods for the recycling of NAD(P)(H) cofactors are discussed. In particular, improvements achieved by protein or reaction engineering are presented, as well as new promising regeneration systems, such as those employing the use of hydrogenases, NAD(P)H oxidases, and mediator-coupled strategies, which could further boost the application of NAD(P)H-dependent dehydrogenases toward the development of selective and green catalytic oxidoreductive methods.