Purification and immobilization of the inducible form of extracellular laccase of the fungus trametes versicolor



The inducible form of extracellular laccase (EC produced by Trametes versicolor culture in a 10 1 glass fermenter was isolated and purified by ion-exchange chromatography on the DEAE-Sephadex A-50 column and by affinity chromatography on a syringyl-CPG column. The enzyme was further immobilized on functionalized porous glass. The paper also discusses some properties of immobilized and free enzyme, respectively.