Purification and immobilization of the inducible form of extracellular laccase of the fungus trametes versicolor

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Abstract

The inducible form of extracellular laccase (EC 1.10.3.2) produced by Trametes versicolor culture in a 10 1 glass fermenter was isolated and purified by ion-exchange chromatography on the DEAE-Sephadex A-50 column and by affinity chromatography on a syringyl-CPG column. The enzyme was further immobilized on functionalized porous glass. The paper also discusses some properties of immobilized and free enzyme, respectively.

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