Acknowledgements: We acknowledge Dr. Frank Uhlmann for hosting our biological experiments, Dr. Ina Weisswange for imaging advice, Emica Coric and Anne Weston for SEM measurements and Guillermo Menendez and Anna Wade for kindly providing us with primary mouse neurons. This work was financially supported by a grant from the Volkswagen Foundation (I/84 072) and partial support from the Germany/Hong Kong Joint Research Scheme (Grant No. 426/hk-PPP-cab). MK is supported by funds from a Wellcome Trust University Award (077429/Z/05/Z).
Morphological Differentiation of Neurons on Microtopographic Substrates Fabricated by Rolled-Up Nanotechnology†
Article first published online: 8 OCT 2010
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Engineering Materials
Special Issue: 1st Sino-German Symposium on Advanced Biomedical Nanostructures
Volume 12, Issue 9, pages B558–B564, September, 2010
How to Cite
Schulze, S., Huang, G., Krause, M., Aubyn, D., Quiñones, V. A. B., Schmidt, C. K., Mei, Y. and Schmidt, O. G. (2010), Morphological Differentiation of Neurons on Microtopographic Substrates Fabricated by Rolled-Up Nanotechnology. Adv. Eng. Mater., 12: B558–B564. doi: 10.1002/adem.201080023
- Issue published online: 27 OCT 2010
- Article first published online: 8 OCT 2010
- Manuscript Revised: 13 MAY 2010
- Manuscript Received: 3 FEB 2010
- Bio-analysis, 3D cell culture, Rolled-up nanotechnology
Arrays of transparent rolled-up microtubes can easily be mass-produced using a combination of conventional photolithography, electron beam depositioning, and chemical etching techniques. Here, we culture primary mouse motor neurons and immortalised CAD cells, a cell line derived from the central nervous system, on various microtube substrates to investigate the influence of topographical surface features on the growth and differentiation behaviour of these cells. Our results indicate that the microtube chips not only support growth of both cell types but also provide a well-defined, geometrically confined 3D cell culture scaffold. Strikingly, our micropatterns act as a platform for axon guidance with protruding cell extensions aligning in the direction of the microtubes and forming complex square-shaped grid-like neurite networks. Our experiments open up a cost-efficient and bio-compatible way of analysing single cell behaviour in the context of advanced micro-/nanostructures with various biological applications ranging from neurite protection studies to cell sensor development.