Author would like to acknowledge Prof. M. Sen, Dr. T. Kohler, Dr. I. Wimpenny, Mr. C. Bayram and Mr. M. Demirbilek for their help in the analyses and proof-reading of the manuscript. Polymers were kindly donated by Purac, Netherlands. This project was partly supported by European Union FP7 IEF Marie Curie Actions (PIEF-GA-2009-237762) and BMT Calsis, Turkey.
A Three-Layered Osteochondral Plug: Structural, Mechanical, and in vitro Biocompatibility Analysis†
Article first published online: 30 JUN 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Engineering Materials
Volume 13, Issue 12, pages B511–B517, December 2011
How to Cite
Aydin, H. M. (2011), A Three-Layered Osteochondral Plug: Structural, Mechanical, and in vitro Biocompatibility Analysis. Adv. Eng. Mater., 13: B511–B517. doi: 10.1002/adem.201180005
- Issue published online: 7 DEC 2011
- Article first published online: 30 JUN 2011
- Manuscript Revised: 15 APR 2011
- Manuscript Received: 24 JAN 2011
This study aims to produce an osteochondral plug with three distinct layers resembling the naturally occurring cartilage, tidemark, and subchondral zones, for the regeneration of defects of articular cartilage. The bone layer is constructed from a PLLA/PCL polymeric blend using a dual-porogen approach. The pore surfaces are coated with type-I collagen and hydroxyapatite. The upper layer, made of PGA nonwoven felt, is combined with the lower using a polymeric blend with a pigment for better visualization during implantation. Vertical channels are formed from the bottom layer to the upper border of the tidemark to facilitate the delivery of stem cells and blood from the bone marrow when implanted. The Young's modulus of the osteochondral plugs is 94.5 ± 9.42 kPa. Cell-culture studies confirm the biocompatibility.