This work was partially supported by the bilateral Russian-French program (the joint CNRS-RFBR PICS project N. 10-04-91056) and also by RFBR project N. 10-03-01022. The authors are grateful to Dr. J. McGuire (Roswell Park Cancer Institute, Buffalo, USA) for the kind gift of human leukemia cell lines and to Dr. T. Erokhina (Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of RAS, Russia) for mouse myeloma Sp2/0 cells. The authors are also thankful to Prof. E. Vodovozova (Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of RAS, Russia) and to Prof. A. Bartkowiak (Westpomeranian University of Technology, Poland) for their kind support of this research.
Biocompatible Smart Microcapsules Based on Chitosan-Poly(vinyl alcohol) Copolymers for Cultivation of Animal Cells†
Article first published online: 30 JUN 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Advanced Engineering Materials
Volume 13, Issue 12, pages B493–B503, December 2011
How to Cite
Zaytseva-Zotova, D., Balysheva, V., Tsoy, A., Drozdova, M., Akopova, T., Vladimirov, L., Chevalot, I., Marc, A., Goergen, J.-L. and Markvicheva, E. (2011), Biocompatible Smart Microcapsules Based on Chitosan-Poly(vinyl alcohol) Copolymers for Cultivation of Animal Cells. Adv. Eng. Mater., 13: B493–B503. doi: 10.1002/adem.201180014
- Issue published online: 7 DEC 2011
- Article first published online: 30 JUN 2011
- Manuscript Revised: 15 APR 2011
- Manuscript Received: 1 MAR 2011
In this study, two novel chitosan-graft-poly(vinyl alcohol) copolymers are synthesized and used as water-soluble at physiological conditions polycations for preparation of smart microcapsules. The microcapsules provide growth and proliferation of eight mammalian cell lines, including hybridoma and tumor cells, at long-term cell cultivation in vitro. The microcapsules are stable in cell culture medium but can be dissolved by changing pH value of the medium (up to 8.0–8.2), thus making possible a simple release of the entrapped cells. Monoclonal antibody production by encapsulated hybridoma cells is demonstrated. Cultivation of tumor cells within the microcapsules allows the formation of 3D multicellular spheroids, which can be proposed as an in vitro model for anticancer drug screening.